Bengtsson A, Redl H, Schlag G, Mollnes T E, Högåsen K
Department of Anaesthesiology & Intensive Care, Sahlgrenska University Hospital, Göteborg, Sweden.
Acta Anaesthesiol Scand. 1996 Feb;40(2):244-9. doi: 10.1111/j.1399-6576.1996.tb04427.x.
Sepsis and septic shock lead to activation of the complement cascade and to the release of pro-inflammatory cytokines.
The effects of E coli infusion and of infusion of anti-TNF antibodies and a xanthine derivative (HWA 138) on complement activation and cytokine release was evaluated in 17 baboons. All animals received 5 x 10(8) live bacteria per kg body weight. Five animals received only bacteria, five received in addition 0.5 mg per kg body weight of anti-TNF-antibody, and seven received an infusion of 6 mg per kg body weight of HWA 138 in addition to the bacteria.
In baboons receiving 5 x 10(8) live E coli per kg body weight increased plasma levels of TCC, TNF-alpha and IL-8 were found. The release of TNF-alpha was lower in the group receiving HWA 138 at 2 h after the infusion. In baboons receiving an infusion of anti-TNF antibody the concentration of IL-8 was lower at 2 and 4 h than in animals receiving just E coli or HWA 138.
Infusion of anti-TNF-antibody before E coli infusion will decrease the formation of IL-8. Infusion of HWA 138 before the E coli infusion will also inhibit the formation of TNF-alpha.
脓毒症和感染性休克会导致补体级联反应激活以及促炎细胞因子释放。
在17只狒狒中评估了输注大肠杆菌、抗TNF抗体和一种黄嘌呤衍生物（HWA 138）对补体激活和细胞因子释放的影响。所有动物每千克体重接受5×10⁸ 活细菌。5只动物仅接受细菌，5只动物还额外接受每千克体重0.5毫克抗TNF抗体，7只动物除细菌外还接受每千克体重6毫克HWA 138的输注。
在每千克体重接受5×10⁸ 活大肠杆菌的狒狒中，发现血浆中TCC、TNF-α和IL-8水平升高。输注后2小时，接受HWA 138的组中TNF-α的释放较低。在接受抗TNF抗体输注的狒狒中，2小时和4小时时IL-8的浓度低于仅接受大肠杆菌或HWA 138的动物。
在输注大肠杆菌之前输注抗TNF抗体将减少IL-8的形成。在输注大肠杆菌之前输注HWA 138也将抑制TNF-α的形成。
