宽视场下秀丽隐杆线虫的彩色和黑白无透镜片上成像。
Color and monochrome lensless on-chip imaging of Caenorhabditis elegans over a wide field-of-view.
机构信息
Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
出版信息
Lab Chip. 2010 May 7;10(9):1109-12. doi: 10.1039/c001200a. Epub 2010 Mar 19.
Abstract
We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lensless in-line holography. Digital reconstruction of the recorded lensless holograms rapidly creates the C. elegans images within <1 s over a field-of-view of >24 mm2. By digitally combining the reconstructed images at three different wavelengths (red, green and blue), color images of dyed samples are also acquired. This wide field-of-view and compact on-chip imaging modality also permits straightforward integration with microfluidic systems.
摘要
我们展示了使用非相干无透镜共线全息术对秀丽隐杆线虫样本进行宽视场彩色和黑白成像。通过对记录的无透镜全息图进行数字重建，在 >24 mm2 的视场范围内，可在 <1 s 内快速生成秀丽隐杆线虫的图像。通过在三个不同波长（红、绿和蓝）下对重建图像进行数字组合，还可以获得染色样本的彩色图像。这种宽视场和紧凑的芯片内成像方式还允许与微流控系统进行直接集成。
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