Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
IEEE Rev Biomed Eng. 2013;6:29-46. doi: 10.1109/RBME.2012.2215847.
Lab-on-a-chip systems have been rapidly emerging to pave the way toward ultra-compact, efficient, mass producible and cost-effective biomedical research and diagnostic tools. Although such microfluidic and microelectromechanical systems have achieved high levels of integration, and are capable of performing various important tasks on the same chip, such as cell culturing, sorting and staining, they still rely on conventional microscopes for their imaging needs. Recently, several alternative on-chip optical imaging techniques have been introduced, which have the potential to substitute conventional microscopes for various lab-on-a-chip applications. Here we present a critical review of these recently emerging on-chip biomedical imaging modalities, including contact shadow imaging, lens-free holographic microscopy, fluorescent on-chip microscopy and lens-free optical tomography.
微流控芯片系统的出现为超紧凑、高效、大规模生产和具有成本效益的生物医学研究和诊断工具铺平了道路。虽然这种微流控和微机电系统已经实现了高度集成，并能够在同一张芯片上执行各种重要任务，如细胞培养、分选和染色，但它们仍然依赖于传统显微镜来满足其成像需求。最近，已经引入了几种替代的芯片上光学成像技术，这些技术有可能替代传统显微镜在各种微流控芯片应用中的地位。在这里，我们对这些新兴的芯片上生物医学成像模式进行了批判性的综述，包括接触阴影成像、无透镜全息显微镜、芯片上荧光显微镜和无透镜光学层析成像。
