Food Science and Technology Institute, Federal University of Rio Grande do Sul State, Porto Alegre, RS, Brazil.
J Environ Sci Health B. 2010 Jan;45(1):67-72. doi: 10.1080/03601230903404499.
The objective of this work was to isolate bacteria from soil historically exposed to tebuconazole and to evaluate the biodegradation of this fungicide by them. Tebuconazole is a commonly used systemic fungicide of the triazol group, which inhibits the sterol C-14 alpha-demethylation of 24-methylenedihydrolanosterol, a precursor of ergosterol, a cell membrane component in fungi. Microorganisms were isolated by different methods of soil sampling and the screening of degrading bacteria was performed in bioreactors cultivations, with some isolates showing the ability to degrade up to 42.76 mg L(- 1) of tebuconazole (51% of the initial concentration). These strains were identified by standard biochemical procedures as being Enterobacter sakazakii and Serratia sp. These bacteria present some important characteristics for potential uses on environmental bioremediation, considering that tebucanozale is an extremely recalcitrant chemical.
本工作的目的是从历史上接触过戊唑醇的土壤中分离细菌，并评估它们对这种杀菌剂的生物降解能力。戊唑醇是一种常用的三唑类内吸性杀菌剂，它抑制 24-亚甲基二氢羊毛甾醇的 C-14α-脱甲基化，24-亚甲基二氢羊毛甾醇是麦角甾醇的前体，麦角甾醇是真菌细胞膜的组成部分。通过不同的土壤采样方法分离微生物，并在生物反应器培养物中进行降解菌的筛选，一些分离物显示出能够降解高达 42.76 毫克/升（初始浓度的 51%）的戊唑醇的能力。这些菌株通过标准生化程序鉴定为阪崎肠杆菌和沙雷氏菌属。这些细菌具有一些重要的特性，考虑到戊唑醇是一种极其难降解的化学物质，它们可能用于环境生物修复。
