Anuradha B, Rekhapadmini A, Rangaswamy V
Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, 515 003, India.
3 Biotech. 2016 Jun;6(1):78. doi: 10.1007/s13205-016-0367-0. Epub 2016 Feb 23.
The efficacy of two selected fungicides i.e., tebuconazole and coppoer hydroxide, was conducted experiments in laboratory and copper hydroxide on the two specific enzymes phosphatase and urease were determined in two different soil samples (red sandy loam and black clay soils) of groundnut (Arachis hypogaea L.) from cultivated fields of Anantapuramu District, Andhra Pradesh. The activities of the selected soil enzymes were determined by incubating the selected fungicides-treated (1.0, 2.5, 5.0, 7.5 and 10.0 kg ha) and -untreated groundnut soil samples at 10 day intervals. By determining the effective concentration, the rate of selected enzyme activity was estimated by adding the suitable substrate at 10, 20, 30 and 40 days of soil incubation. Both the enzyme activities were increased up to 5.0 kg ha level of fungicide in both soil samples significantly at 10 days of soil incubation and further enhanced up to 20 days of incubation. The activity of the phosphatase and urease decreased progressively at 30 and 40 days of incubation. From overall studies, higher concentrations (7.5 and 10.0 kg ha) of both tebuconazole and copper hydroxide were toxic to phosphatase and urease activities, respectively, in both soil samples.
在实验室中对两种选定的杀菌剂戊唑醇和氢氧化铜的功效进行了试验，并在安得拉邦阿南塔普拉姆区耕地上的花生（落花生）的两种不同土壤样本（红砂壤土和黑粘土）中测定了氢氧化铜对两种特定酶磷酸酶和脲酶的影响。通过每隔10天培养选定杀菌剂处理过的（1.0、2.5、5.0、7.5和10.0千克/公顷）和未处理的花生土壤样本，来测定选定土壤酶的活性。通过在土壤培养的第10、20、30和40天添加合适的底物来确定有效浓度，从而估算选定酶活性的速率。在土壤培养10天时，两种土壤样本中两种酶的活性在杀菌剂浓度达到5.0千克/公顷水平时均显著增加，并在培养至20天时进一步增强。在培养30天和40天时，磷酸酶和脲酶的活性逐渐下降。从总体研究来看，戊唑醇和氢氧化铜的较高浓度（7.5和10.0千克/公顷）分别对两种土壤样本中的磷酸酶和脲酶活性有毒害作用。
