Institute of Water Supply and Environmental Protection, Cracow University of Technology, Krakow, Poland.
J Environ Sci Health B. 2010 Jan;45(1):82-8. doi: 10.1080/03601230903404572.
Methanogenic granular sludge and wastewater fermented sludge were used as inocula for batch tests of anaerobic bioremediation of chlorinated pesticide contaminated soil. Results obtained for both types of biomass were similar: 80 to over 90% of gamma -hexachlorocyclohexane (gamma-HCH), 1,1,1-trichloro-2,2-bis-(4-methoxyphenyl)ethane (methoxychlor) and 1,1,1-trichloro-2,2-bis-(4-chlorophenyl)ethane (DDT) removed in 4-6 weeks. Residual fractions of these pesticides persisted till the end of the 16-week experiment. DDT was degraded through 1,1-dichloro-2,2-bis-(4-chlorophenyl)ethane (DDD). Accumulation of this product corresponded stoichiometrically only to 34-53% of removed DDT, supposedly due to its further transformations, finally resulting in formation of detected 4,4'-dichlorobenzophenone (DBP). Addition of 0.5 mM Tween 80 nonionic surfactant resulted in about a twofold decrease of gamma -HCH and methoxychlor residual concentrations, as well as considerably lower DDD accumulation (7-29%) and higher DBP production. However, 1.25 mM dose of this surfactant applied together with granular sludge brought DDD levels back to that observed for treatments with the sludge alone, also impairing DBP formation.
采用产甲烷颗粒污泥和废水发酵污泥作为接种物，进行了氯代农药污染土壤的厌氧生物修复批式试验。两种生物量的结果相似：γ-六氯环己烷（γ-HCH）、1,1,1-三氯-2,2-双（4-甲氧基苯基）乙烷（甲氧氯）和 1,1,1-三氯-2,2-双（4-氯苯基）乙烷（DDT）的去除率均在 80%以上，在 4-6 周内去除率超过 90%。这些农药的残留部分在 16 周的实验结束时仍然存在。DDT 通过 1,1-二氯-2,2-双（4-氯苯基）乙烷（DDD）降解。该产物的积累仅与去除的 DDT 的 34-53% 相当，据推测是由于其进一步转化，最终导致检测到的 4,4'-二氯二苯甲酮（DBP）的形成。添加 0.5mM Tween 80 非离子表面活性剂可使γ-HCH 和甲氧氯的残留浓度降低约两倍，同时 DDD 的积累（7-29%）较低，DBP 的产量较高。然而，与颗粒污泥一起施加 1.25mM 剂量的这种表面活性剂会使 DDD 水平恢复到仅用污泥处理时观察到的水平，同时也会损害 DBP 的形成。
