基于氙气的分子传感器组装在 MS2 病毒衣壳支架上。

A xenon-based molecular sensor assembled on an MS2 viral capsid scaffold.

机构信息

College of Chemistry, University of California, Berkeley, Berkeley, California 94720, USA.

出版信息

J Am Chem Soc. 2010 May 5;132(17):5936-7. doi: 10.1021/ja100319f.

PMID:20392049

Abstract

In MRI, anatomical structures are most often differentiated by variations in their bulk magnetic properties. Alternatively, exogenous contrast agents can be attached to chemical moieties that confer affinity to molecular targets; the distribution of such contrast agents can be imaged by magnetic resonance. Xenon-based molecular sensors are molecular imaging agents that rely on the reversible exchange of hyperpolarized xenon between the bulk and a specifically targeted host-guest complex. We have incorporated approximately 125 xenon sensor molecules in the interior of an MS2 viral capsid, conferring multivalency and other properties of the viral capsid to the sensor molecule. The resulting signal amplification facilitates the detection of sensor at 0.7 pM, the lowest to date for any molecular imaging agent used in magnetic resonance. This amplification promises the detection of chemical targets at much lower concentrations than would be possible without the capsid scaffold.

摘要

在 MRI 中，解剖结构通常通过其体磁性质的变化来区分。或者，可以将外源性对比剂附着在赋予与分子靶标亲和力的化学部分上；通过磁共振成像可以对这些对比剂的分布进行成像。氙基分子传感器是一种分子成像剂，它依赖于超极化氙气在主体和特定靶向主体-客体配合物之间的可逆交换。我们已经将大约 125 个氙气传感器分子纳入 MS2 病毒衣壳的内部，赋予传感器分子多价性和病毒衣壳的其他性质。由此产生的信号放大有助于在 0.7 pM 时检测到传感器，这是迄今为止在磁共振中使用的任何分子成像剂中检测到的最低浓度。这种放大有望在没有衣壳支架的情况下，以比以前更低的浓度检测到化学靶标。