GlaxoSmithKline, 5 Moore Drive, Research Triangle Park, North Carolina 27709, USA.
Curr Gene Ther. 2010 Jun;10(3):168-73. doi: 10.2174/156652310791321224.
Modern drug discovery programs utilize a wide variety of technologies to aid in identification of potential drug targets, and progress them through the often long and winding path of finding novel drug-like molecules. Recombinant cell-based assays are an important tool in the drug discovery process for investigating the biological mechanisms of potential drug targets and conducting screening campaigns in the hunt for biologically active molecules. Historically, stable cell lines expressing the target protein(s) of interest have been used for these assays. Although such cell lines can be useful, their development can be laborious and the resulting cell line affords little experimental flexibility. Transient gene expression approaches provide an alternative to the often tedious task of developing and maintaining numerous stable cell lines. Recently the unique properties of modified baculoviruses, containing mammalian expression cassettes and referred to as BacMam viruses, have been exploited to facilitate rapid and reproducible transient cell-based assay development. This review will focus on the many features of BacMam virus gene delivery that make it a powerful system for cell-based assay development and screening.
现代药物发现计划利用各种技术来辅助鉴定潜在的药物靶点，并通过通常漫长而曲折的途径来寻找新的类似药物的分子。重组细胞测定法是药物发现过程中用于研究潜在药物靶点的生物学机制并进行筛选以寻找具有生物活性的分子的重要工具。从历史上看，表达感兴趣的靶蛋白的稳定细胞系已用于这些测定。尽管这些细胞系可能很有用，但它们的开发可能很费力，并且所得的细胞系几乎没有实验灵活性。瞬时基因表达方法为开发和维护众多稳定细胞系这一通常繁琐的任务提供了替代方法。最近，已利用含有哺乳动物表达盒的改良杆状病毒（称为 BacMam 病毒）的独特特性来促进快速且可重复的瞬时基于细胞的测定法的开发。本文将重点介绍 BacMam 病毒基因传递的许多功能，这些功能使其成为基于细胞的测定法开发和筛选的强大系统。
