棒状杆菌中质粒分配功能的鉴定
Identification of plasmid partition function in coryneform bacteria.
作者信息
Kurusu Y, Satoh Y, Inui M, Kohama K, Kobayashi M, Terasawa M, Yukawa H
机构信息
Tsukuba Research Center, Mitsubishi Petrochemical Co., Ltd., Ibaraki, Japan.
出版信息
Appl Environ Microbiol. 1991 Mar;57(3):759-64. doi: 10.1128/aem.57.3.759-764.1991.
Abstract
We have identified and characterized a partition function that is required for stable maintenance of plasmids in the coryneform bacteria Brevibacterium flavum MJ233 and Corynebacterium glutamicum ATCC 31831. This function is localized to a HindIII-NspV fragment (673 bp) adjacent to the replication region of the plasmid, named pBY503, from Brevibacterium stationis IFO 12144. The function was independent of copy number control and was not associated directly with plasmid replication functions. This fragment was able to stabilize the unstable plasmids in cis but not in trans.
摘要
我们已经鉴定并表征了一种分配功能，该功能对于在棒状细菌黄色短杆菌MJ233和谷氨酸棒杆菌ATCC 31831中稳定维持质粒是必需的。该功能定位于来自停滞短杆菌IFO 12144的质粒pBY503复制区域附近的一个HindIII - NspV片段（673 bp）。该功能独立于拷贝数控制，并且不直接与质粒复制功能相关。这个片段能够顺式稳定不稳定的质粒，但不能反式稳定。
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