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1
Cloning vector system for Corynebacterium glutamicum.谷氨酸棒杆菌的克隆载体系统
J Bacteriol. 1985 May;162(2):591-7. doi: 10.1128/jb.162.2.591-597.1985.
2
A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing.用于克隆、可控基因表达和启动子探测的谷氨酸棒杆菌/大肠杆菌穿梭载体家族。
Gene. 1991 Jun 15;102(1):93-8. doi: 10.1016/0378-1119(91)90545-m.
3
The erythromycin resistance gene of the Corynebacterium xerosis R-plasmid pTP10 also carrying chloramphenicol, kanamycin, and tetracycline resistances is capable of transposition in Corynebacterium glutamicum.干燥棒状杆菌R质粒pTP10的红霉素抗性基因还携带氯霉素、卡那霉素和四环素抗性，该基因能够在谷氨酸棒状杆菌中发生转座。
Plasmid. 1995 May;33(3):168-79. doi: 10.1006/plas.1995.1018.
4
Structural organization of the Corynebacterium glutamicum plasmid pCG100.谷氨酸棒杆菌质粒pCG100的结构组织
J Gen Microbiol. 1991 Sep;137(9):2093-101. doi: 10.1099/00221287-137-9-2093.
5
[Molecular cloning and the expression of the genes of amino acid biosynthesis of Corynebacterium glutamicum in Escherichia coli cells].[谷氨酸棒杆菌氨基酸生物合成基因在大肠杆菌细胞中的分子克隆与表达]
Genetika. 1988 Jul;24(7):1153-8.
6
Cloning restriction fragments that promote expression of a gene in Bacillus subtilis.克隆促进基因在枯草芽孢杆菌中表达的限制性片段。
J Bacteriol. 1981 Jun;146(3):1162-5. doi: 10.1128/jb.146.3.1162-1165.1981.
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[Development of the vector-host system in Corynebacterium. Cloning and expression of homoserine dehydrogenase and homoserine kinase genes in Corynebacterium cells].[棒状杆菌载体-宿主系统的开发。高丝氨酸脱氢酶和高丝氨酸激酶基因在棒状杆菌细胞中的克隆与表达]
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8
A shuttle vector system for Brevibacterium lactofermentum.用于乳酸发酵短杆菌的穿梭载体系统。
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9
Construction of novel shuttle vectors and a cosmid vector for the glutamic acid-producing bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum.用于产谷氨酸细菌乳酸发酵短杆菌和谷氨酸棒杆菌的新型穿梭载体和黏粒载体的构建。
Gene. 1985;39(2-3):281-6. doi: 10.1016/0378-1119(85)90324-5.
10
Characterization of the natural deletion mutant of plasmid pXZ10145 in Corynebacterium glutamicum and construction of a recombinant plasmid.谷氨酸棒杆菌中质粒pXZ10145天然缺失突变体的表征及重组质粒的构建
Chin J Biotechnol. 1991;7(4):271-7.

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A chromosomally encoded T7 RNA polymerase-dependent gene expression system for Corynebacterium glutamicum: construction and comparative evaluation at the single-cell level.一种用于谷氨酸棒杆菌的染色体编码的依赖T7 RNA聚合酶的基因表达系统：单细胞水平的构建与比较评估。
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7
Plasmid pGA1 from Corynebacterium glutamicum codes for a gene product that positively influences plasmid copy number.来自谷氨酸棒杆菌的质粒pGA1编码一种对质粒拷贝数有正向影响的基因产物。
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8
Stable transformation of the gram-positive phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus with several cloning vectors.用多种克隆载体对革兰氏阳性植物致病细菌密歇根棒杆菌番茄溃疡病菌亚种进行稳定转化。
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9
Mutations in the trpD gene of Corynebacterium glutamicum confer 5-methyltryptophan resistance by encoding a feedback-resistant anthranilate phosphoribosyltransferase.谷氨酸棒杆菌trpD基因中的突变通过编码一种抗反馈的邻氨基苯甲酸磷酸核糖基转移酶赋予对5-甲基色氨酸的抗性。
Appl Environ Microbiol. 1995 Dec;61(12):4477-9. doi: 10.1128/aem.61.12.4477-4479.1995.
10
Metabolism of the herbicide atrazine by Rhodococcus strains.红球菌菌株对除草剂阿特拉津的代谢作用
Appl Environ Microbiol. 1993 Jun;59(6):1955-9. doi: 10.1128/aem.59.6.1955-1959.1993.

本文引用的文献

1
Steroid transformation at high substrate concentrations using immobilized Corynebacterium simplex cells.使用固定化简单棒状杆菌细胞在高底物浓度下进行类固醇转化。
Biotechnol Bioeng. 1980 Jan;22(1):119-36. doi: 10.1002/bit.260220110.
2
A survey of the structures of mycolic acids in Corynebacterium and related taxa.棒状杆菌及相关分类群中分枝菌酸结构的研究。
J Gen Microbiol. 1982 Jan;128(1):129-49. doi: 10.1099/00221287-128-1-129.
3
R plasmids in Corynebacterium xerosis strains.干燥棒状杆菌菌株中的R质粒
Antimicrob Agents Chemother. 1983 Mar;23(3):506-8. doi: 10.1128/AAC.23.3.506.
4
Fatty acid composition of some mycolic acid-containing coryneform bacteria.一些含分枝菌酸棒状杆菌的脂肪酸组成
J Gen Microbiol. 1982 Nov;128(11):2503-9. doi: 10.1099/00221287-128-11-2503.
5
Numerical classification of some Rhodococci, Corynebacteria and related organisms.部分红球菌、棒状杆菌及相关微生物的数值分类
J Gen Microbiol. 1982 Apr;128(4):731-45. doi: 10.1099/00221287-128-4-731.
6
The plant pathogenic corynebacteria.植物病原性棒状杆菌。
Annu Rev Microbiol. 1982;36:495-517. doi: 10.1146/annurev.mi.36.100182.002431.
7
Promoter-probe plasmid for Bacillus subtilis.枯草芽孢杆菌启动子探针质粒
J Bacteriol. 1984 Mar;157(3):965-7. doi: 10.1128/jb.157.3.965-967.1984.
8
Genetic transposition and insertional mutagenesis in Bacillus subtilis with Streptococcus faecalis transposon Tn917.利用粪肠球菌转座子Tn917对枯草芽孢杆菌进行基因转座和插入诱变。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2305-9. doi: 10.1073/pnas.80.8.2305.
9
Plasmids in Corynebacterium diphtheriae and diphtheroids mediating erythromycin resistance.介导红霉素耐药的白喉棒状杆菌和类白喉杆菌中的质粒
Antimicrob Agents Chemother. 1980 Nov;18(5):814-21. doi: 10.1128/AAC.18.5.814.
10
Protoplast transformation of glutamate-producing bacteria with plasmid DNA.用质粒DNA对谷氨酸生产菌进行原生质体转化。
J Bacteriol. 1984 Jul;159(1):306-11. doi: 10.1128/jb.159.1.306-311.1984.

谷氨酸棒杆菌的克隆载体系统

Cloning vector system for Corynebacterium glutamicum.

作者信息

Yoshihama M, Higashiro K, Rao E A, Akedo M, Shanabruch W G, Follettie M T, Walker G C, Sinskey A J

出版信息

J Bacteriol. 1985 May;162(2):591-7. doi: 10.1128/jb.162.2.591-597.1985.

DOI:10.1128/jb.162.2.591-597.1985
PMID:3921526
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC218889/
Abstract

A protoplast transformation system has been developed for Corynebacterium glutamicum by using a C. glutamicum-Bacillus subtilis chimeric vector. The chimera was constructed by joining a 3.0-kilobase cryptic C. glutamicum plasmid and the B. subtilis plasmid pBD10. The neomycin resistance gene on the chimera, pHY416, was expressed in C. glutamicum, although the chloramphenicol resistance gene was not. The various parameters in the transformation protocol were analyzed separately and optimized. The resulting transformation system is simple and routinely yields 10(4) transformants per microgram of plasmid DNA.

摘要

通过使用谷氨酸棒杆菌-枯草芽孢杆菌嵌合载体，已开发出一种谷氨酸棒杆菌原生质体转化系统。该嵌合体通过连接一个3.0千碱基的谷氨酸棒杆菌隐蔽质粒和枯草芽孢杆菌质粒pBD10构建而成。嵌合体pHY416上的新霉素抗性基因在谷氨酸棒杆菌中表达，而氯霉素抗性基因则不表达。对转化方案中的各种参数进行了单独分析和优化。所得的转化系统简单，每微克质粒DNA通常可产生10⁴个转化体。