Institute of Clinical Immunology, University of Los Andes, Merida, Venezuela.
Cell Immunol. 2010;263(2):166-71. doi: 10.1016/j.cellimm.2010.03.012. Epub 2010 Mar 27.
Altered neutrophil function may contribute to the development of AIDS during the course of HIV infection. It has been described that Nef, a regulatory protein from HIV, can modulate superoxide production in other cells, therefore altered superoxide production in neutrophils from HIV infected patients, could be secondary to a direct effect of Nef on components of the NADPH oxidase complex. In this work, we describe that Nef, was capable of increasing superoxide production in human neutrophils. Furthermore, a specific association between Nef and p22-phox, a membrane component of the NADPH oxidase complex, was found. We propose that this association may reflect a capability of Nef to modulate by direct association, the enzymatic complex responsible for one of the most efficient innate defense mechanisms in phagocytes, contributing to the pathogenesis of the disease.
中性粒细胞功能的改变可能有助于 HIV 感染过程中 AIDS 的发展。已经描述了 HIV 的调节蛋白 Nef 可以调节其他细胞中超氧化物的产生，因此 HIV 感染患者中性粒细胞中超氧化物产生的改变可能是 Nef 对 NADPH 氧化酶复合物成分的直接作用的结果。在这项工作中，我们描述了 Nef 能够增加人中性粒细胞中超氧化物的产生。此外，还发现了 Nef 与 NADPH 氧化酶复合物的膜成分 p22-phox 之间的特定关联。我们提出，这种关联可能反映了 Nef 通过直接关联来调节负责吞噬细胞中最有效先天防御机制之一的酶复合物的能力，从而有助于疾病的发病机制。
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