Soeda E, Kimura G, Miura K
Proc Natl Acad Sci U S A. 1978 Jan;75(1):162-6. doi: 10.1073/pnas.75.1.162.
The nucleotide sequence around the origin of replication in DNA of mouse polyoma virus was determined by 32P labeling of the 3' terminus of the Hap II-5/Alu I-1 DNA fragment, with the use of DNA polymerase. The result coincided with our previous report on the 32P labeling, with the use of polynucleotide kinase, of the 5' terminus of the Hap II-5/Hha I-1 DNA fragment, which corresponds to the large part of the present fragment, Hap II-5/Alu I-1. A symmetrical (A+T)-rich region containing a five-A stretch (or a five-T stretch) was flanked by two small regions with a 2-fold rotational axis of symmetry. On comparison of the sequence near the replication origin of polyoma DNA with that in the corresponding region of simian virus 40 DNA, which was included in the EcoRI-G fragment sequenced by Weissman's group (Subramanian K.N., Dahr, R. & Weissman, S. M. (1977) J. Biol. Chem. 252, 355--367), a considerable similarity was detected. Several possible common sequences for important biological activities such as the starting of DNA replication and RNA synthesis were suggested.
利用DNA聚合酶对小鼠多瘤病毒DNA中复制起点周围的核苷酸序列进行了测定，方法是对Hap II - 5/Alu I - 1 DNA片段的3'末端进行32P标记。该结果与我们之前关于利用多核苷酸激酶对Hap II - 5/Hha I - 1 DNA片段（相当于当前片段Hap II - 5/Alu I - 1的大部分）的5'末端进行32P标记的报告一致。一个含有五个A序列（或五个T序列）的对称（富含A+T）区域两侧是两个具有2倍对称轴的小区域。将多瘤病毒DNA复制起点附近的序列与猴病毒40 DNA相应区域的序列（该序列包含在魏斯曼小组测序的EcoRI - G片段中，Subramanian K.N., Dahr, R. & Weissman, S.M. (1977) J. Biol. Chem. 252, 355 - 367）进行比较，发现了相当大的相似性。提出了几种可能的重要生物学活性共同序列，如DNA复制起始和RNA合成。
