由限制性核酸内切酶产生的SV40 DNA片段的图谱绘制与排序。
The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases.
作者信息
Subramanian K N, Pan J, Zain S, Weissman S M
出版信息
Nucleic Acids Res. 1974 Jun;1(6):727-52. doi: 10.1093/nar/1.6.727.
SV40 DNA is cleaved by the Eco RII and Hae restriction endonucleases to give rise to two different sets of 16 fragments each. These fragments have been ordered by analysis of the products of redigestion of one set of fragments with another restriction enzyme. The cleavage sites have been precisely mapped based on length measurements of the products of each cleavage. This provides a convenient group of small DNA fragments suitable for sequence analysis investigation of the transcripts present in infected cells, or construction of deletion substitution variants of the virus.
猿猴病毒40（SV40）的DNA被Eco RII和Hae限制性内切酶切割，分别产生两组不同的16个片段。通过用另一种限制酶对一组片段进行再消化产物的分析，这些片段已被排序。基于每次切割产物的长度测量，切割位点已被精确绘制。这提供了一组方便的小DNA片段，适用于对感染细胞中存在的转录本进行序列分析研究，或构建病毒的缺失替代变体。
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