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由限制性核酸内切酶产生的SV40 DNA片段的图谱绘制与排序。

The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases.

作者信息

Subramanian K N, Pan J, Zain S, Weissman S M

出版信息

Nucleic Acids Res. 1974 Jun;1(6):727-52. doi: 10.1093/nar/1.6.727.

DOI:10.1093/nar/1.6.727
PMID:10793753
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343376/
Abstract

SV40 DNA is cleaved by the Eco RII and Hae restriction endonucleases to give rise to two different sets of 16 fragments each. These fragments have been ordered by analysis of the products of redigestion of one set of fragments with another restriction enzyme. The cleavage sites have been precisely mapped based on length measurements of the products of each cleavage. This provides a convenient group of small DNA fragments suitable for sequence analysis investigation of the transcripts present in infected cells, or construction of deletion substitution variants of the virus.

摘要

猿猴病毒40（SV40）的DNA被Eco RII和Hae限制性内切酶切割，分别产生两组不同的16个片段。通过用另一种限制酶对一组片段进行再消化产物的分析，这些片段已被排序。基于每次切割产物的长度测量，切割位点已被精确绘制。这提供了一组方便的小DNA片段，适用于对感染细胞中存在的转录本进行序列分析研究，或构建病毒的缺失替代变体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d12/343376/fb6c6006728e/nar00515-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d12/343376/31681972cdc7/nar00515-0008-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d12/343376/fb6c6006728e/nar00515-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d12/343376/31681972cdc7/nar00515-0008-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d12/343376/fb6c6006728e/nar00515-0015-a.jpg

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本文引用的文献

1
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.一种来自流感嗜血杆菌的限制性内切酶。I. 纯化及一般特性。
J Mol Biol. 1970 Jul 28;51(2):379-91. doi: 10.1016/0022-2836(70)90149-x.
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Recognition sequence of a restriction enzyme.限制酶的识别序列。
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Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.由埃及嗜血杆菌的一种限制酶——核酸内切酶Z产生的φX174脱氧核糖核酸的特定片段。
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Cloning of human satellite III DNA: different components are on different chromosomes.人类卫星III DNA的克隆：不同组分位于不同染色体上。
Nucleic Acids Res. 1979 Jul 25;6(10):3177-97. doi: 10.1093/nar/6.10.3177.
8
A restriction endonuclease cleavage map of mitochondrial DNA from transformed hamster cells.转化仓鼠细胞线粒体DNA的限制性内切酶切割图谱。
Nucleic Acids Res. 1978 Feb;5(2):403-24. doi: 10.1093/nar/5.2.403.
9
A simple and fast electrophoretic method for elution of nucleic acids from gels.一种从凝胶中洗脱核酸的简单快速的电泳方法。
Mol Biol Rep. 1979 Dec 31;5(4):237-9. doi: 10.1007/BF00782896.
10
The nucleotide sequence surrounding the origin of DNA replication of Col E1.大肠杆菌E1 DNA复制起点周围的核苷酸序列。
Nucleic Acids Res. 1977 Sep;4(9):3123-42. doi: 10.1093/nar/4.9.3123.
J Virol. 1972 Jul;10(1):42-50. doi: 10.1128/JVI.10.1.42-50.1972.
4
Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.猴病毒40 DNA的研究。VII。SV40基因组的切割图谱。
J Mol Biol. 1973 Aug 5;78(2):363-76. doi: 10.1016/0022-2836(73)90122-8.
5
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.利用分析型琼脂糖-溴化乙锭电泳检测副流感嗜血杆菌中的两种限制性内切酶活性。
Biochemistry. 1973 Jul 31;12(16):3055-63. doi: 10.1021/bi00740a018.
6
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J Virol. 1973 May;11(5):682-93. doi: 10.1128/JVI.11.5.682-693.1973.
7
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J Virol. 1973 Apr;11(4):508-14. doi: 10.1128/JVI.11.4.508-514.1973.
8
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Virology. 1973 Feb;51(2):517-20. doi: 10.1016/0042-6822(73)90455-8.
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Cleavage of Simian virus 40 DNA at a unique site by a bacterial restriction enzyme.猿猴病毒40 DNA在一个独特位点被一种细菌限制酶切割。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3365-9. doi: 10.1073/pnas.69.11.3365.
10
Specificity of the break produced by restricting endonuclease R 1 in Simian virus 40 DNA, as revealed by partial denaturation mapping.通过部分变性图谱揭示限制内切酶R 1对猿猴病毒40 DNA产生的断裂的特异性。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3215-9. doi: 10.1073/pnas.69.11.3215.