高分辨率熔解曲线分析 7SL RNA 基因对旧大陆利什曼病的快速诊断。

Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene.

机构信息

Department of Microbiology and Molecular Genetics, IMIRC, the Kuvin Center for the Study of Infectious and Tropical Diseases, the Hebrew University-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.

出版信息

J Clin Microbiol. 2010 Jun;48(6):2240-2. doi: 10.1128/JCM.00553-10. Epub 2010 Apr 14.

DOI:10.1128/JCM.00553-10

PMID:20392923

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2884505/

Abstract

High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.

摘要

高分辨率熔解曲线分析 PCR（HRM-PCR）用于诊断旧世界利什曼原虫，使用的是 7SL RNA 基因。分析了皮肤利什曼病样本。HRM-PCR 的敏感性和特异性明显优于内部转录间隔区 1 PCR（P<0.001），与动基体 DNA PCR 相似。