滤纸病变印迹 PCR 对皮肤利什曼病继发感染性溃疡和非溃疡性病变的诊断性能。
Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis.
机构信息
Tropical Disease Unit, Toronto General Hospital, 200 Elizabeth Street, 13th Floor, North Wing, Room 1350, Toronto, ON M5G 1C4, Canada.
出版信息
J Clin Microbiol. 2011 Mar;49(3):1097-100. doi: 10.1128/JCM.02457-10. Epub 2010 Dec 22.
Abstract
We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for secondarily infected lesions (n = 8) were 100%. In primarily nonulcerative lesions (n = 15), the sensitivity of FPLI PCR was inferior to that of pooled-invasive-specimen PCR (72.7% versus 100%) (P = 0.10). FPLI PCR is sensitive, specific, and unlike invasive procedures, can be used in secondarily infected ulcers. Invasive specimen collection is superior in nonulcerative lesions.
摘要
我们比较了传统的皮肤利什曼病诊断方法和滤纸病变印迹（FPLI）PCR 对继发感染性溃疡和非溃疡性病变的检测效果。FPLI PCR 对继发感染性病变（n = 8）的敏感性和特异性均为 100%。在原发性非溃疡性病变（n = 15）中，FPLI PCR 的敏感性低于集合侵入性样本 PCR（72.7%对 100%）（P = 0.10）。FPLI PCR 具有敏感性、特异性，与侵入性操作不同，可用于继发感染性溃疡。在非溃疡性病变中，侵入性标本采集更优。
相似文献
1
Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis.滤纸病变印迹 PCR 对皮肤利什曼病继发感染性溃疡和非溃疡性病变的诊断性能。
J Clin Microbiol. 2011 Mar;49(3):1097-100. doi: 10.1128/JCM.02457-10. Epub 2010 Dec 22.
2
The method used to sample ulcers influences the diagnosis of cutaneous leishmaniasis.用于采集溃疡样本的方法会影响皮肤利什曼病的诊断。
Trans R Soc Trop Med Hyg. 2002 Apr;96 Suppl 1:S169-71. doi: 10.1016/s0035-9203(02)90071-4.
3
PCR performance for the diagnosis of cutaneous leishmaniasis caused by Leishmania viannia complex using biopsy samples, compared with exudate samples from skin lesions on filter paper.聚合酶链反应（PCR）在使用活检样本诊断由利什曼原虫复合种引起的皮肤利什曼病方面的表现，与滤纸皮损渗液样本相比。
Trans R Soc Trop Med Hyg. 2020 Oct 5;114(10):721-724. doi: 10.1093/trstmh/traa052.
4
Detection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis.从美国皮肤利什曼病患者滤纸皮损印迹中检测和鉴定利什曼原虫 DNA。
Clin Infect Dis. 2010 Jan 1;50(1):e1-6. doi: 10.1086/648730.
5
Non-invasive cytology brush PCR for the diagnosis and causative species identification of American cutaneous leishmaniasis in Peru.秘鲁无创性细胞刷 PCR 检测用于美国皮肤利什曼病的诊断和致病种鉴定。
PLoS One. 2012;7(11):e49738. doi: 10.1371/journal.pone.0049738. Epub 2012 Nov 21.
6
Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis.哥伦比亚皮肤利什曼病的诊断：病变内的采样部位会影响寄生虫学诊断的敏感性。
J Clin Microbiol. 2000 Oct;38(10):3768-73. doi: 10.1128/JCM.38.10.3768-3773.2000.
7
Simplified polymerase chain reaction detection of new world Leishmania in clinical specimens of cutaneous leishmaniasis.皮肤利什曼病临床标本中美洲利什曼原虫的简化聚合酶链反应检测
Am J Trop Med Hyg. 1998 Jan;58(1):102-9. doi: 10.4269/ajtmh.1998.58.102.
8
Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene.高分辨率熔解曲线分析 7SL RNA 基因对旧大陆利什曼病的快速诊断。
J Clin Microbiol. 2010 Jun;48(6):2240-2. doi: 10.1128/JCM.00553-10. Epub 2010 Apr 14.
9
Usefulness of sampling with cotton swab for PCR-diagnosis of cutaneous leishmaniasis in the New World.棉拭子采样在新世界皮肤利什曼病PCR诊断中的应用价值。
Acta Trop. 2002 Mar;81(3):197-202. doi: 10.1016/s0001-706x(01)00215-7.
10
American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples.美洲皮肤利什曼病：在非侵入性临床样本中对利什曼原虫进行直接种属鉴定。
Trans R Soc Trop Med Hyg. 2007 Apr;101(4):368-71. doi: 10.1016/j.trstmh.2006.06.009. Epub 2006 Sep 29.
引用本文的文献
1
Diagnostic accuracy of qPCR and microscopy for cutaneous leishmaniasis in rural Ecuador: A Bayesian latent class analysis.基于贝叶斯潜在类别分析的厄瓜多尔农村地区皮肤利什曼病 qPCR 和显微镜检测的诊断准确性。
PLoS Negl Trop Dis. 2023 Nov 29;17(11):e0011745. doi: 10.1371/journal.pntd.0011745. eCollection 2023 Nov.
2
Comparison of Whole Genome Sequencing versus Standard Molecular Diagnostics for Species Identification in the Leishmania Viannia Subgenus.全基因组测序与标准分子诊断在利什曼原虫威尼亚亚属种鉴定中的比较。
Am J Trop Med Hyg. 2021 Jul 16;105(3):660-669. doi: 10.4269/ajtmh.21-0273.
3
Evaluation of conventional and four real-time PCR methods for the detection of Leishmania on field-collected samples in Ethiopia.评估常规和四种实时 PCR 方法在埃塞俄比亚野外采集样本中检测利什曼原虫的效果。
PLoS Negl Trop Dis. 2021 Jan 12;15(1):e0008903. doi: 10.1371/journal.pntd.0008903. eCollection 2021 Jan.
4
Molecular Epidemiological Survey of Cutaneous Leishmaniasis in Two Highly Endemic Metropolises of Iran, Application of FTA Cards for DNA Extraction From Giemsa-Stained Slides.伊朗两个高度流行大都市皮肤利什曼病的分子流行病学调查，FTA卡用于从吉姆萨染色玻片提取DNA的应用。
Jundishapur J Microbiol. 2016 Feb 15;9(2):e32885. doi: 10.5812/jjm.32885. eCollection 2016 Feb.
5
Quantification of Leishmania (Viannia) Kinetoplast DNA in Ulcers of Cutaneous Leishmaniasis Reveals Inter-site and Inter-sampling Variability in Parasite Load.皮肤利什曼病溃疡中利什曼原虫（维扬亚属）动基体DNA的定量分析揭示了寄生虫载量的部位间和采样间变异性。
PLoS Negl Trop Dis. 2015 Jul 23;9(7):e0003936. doi: 10.1371/journal.pntd.0003936. eCollection 2015.
6
Comparison of clinical samples and methods in chronic cutaneous leishmaniasis.慢性皮肤利什曼病临床样本与方法的比较
Am J Trop Med Hyg. 2014 Nov;91(5):895-900. doi: 10.4269/ajtmh.13-0582. Epub 2014 Sep 15.
7
Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR.通过病变拭子采样结合定量聚合酶链反应对皮肤利什曼病进行灵敏诊断。
Parasitology. 2014 Dec;141(14):1891-7. doi: 10.1017/S0031182014001280. Epub 2014 Aug 11.
8
Applicability of kDNA-PCR for routine diagnosis of American tegumentary leishmaniasis in a tertiary reference hospital.kDNA-PCR在三级转诊医院用于美洲皮肤利什曼病常规诊断的适用性
Rev Inst Med Trop Sao Paulo. 2013 Nov-Dec;55(6):393-9. doi: 10.1590/S0036-46652013000600004.
9
The use of fluorescent fragment length analysis (PCR-FFL) in the direct diagnosis and identification of cutaneous Leishmania species.荧光片段长度分析（PCR-FFL）在皮肤利什曼原虫种属的直接诊断和鉴定中的应用。
Am J Trop Med Hyg. 2013 Mar;88(3):586-91. doi: 10.4269/ajtmh.12-0402. Epub 2013 Feb 4.
10
Simultaneous infection with Leishmania (Viannia) braziliensis and L. (V.) lainsoni in a Peruvian patient with cutaneous leishmaniasis.一名秘鲁皮肤利什曼病患者同时感染了巴西利什曼原虫和莱氏利什曼原虫。
Am J Trop Med Hyg. 2013 Apr;88(4):774-7. doi: 10.4269/ajtmh.12-0594. Epub 2013 Feb 4.
本文引用的文献
1
Detection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis.从美国皮肤利什曼病患者滤纸皮损印迹中检测和鉴定利什曼原虫 DNA。
Clin Infect Dis. 2010 Jan 1;50(1):e1-6. doi: 10.1086/648730.
2
First-line therapy for human cutaneous leishmaniasis in Peru using the TLR7 agonist imiquimod in combination with pentavalent antimony.秘鲁采用 TLR7 激动剂咪喹莫特联合五价锑治疗人皮肤利什曼病的一线治疗。
PLoS Negl Trop Dis. 2009 Jul 28;3(7):e491. doi: 10.1371/journal.pntd.0000491.
3
Detection of Leishmania in unaffected mucosal tissues of patients with cutaneous leishmaniasis caused by Leishmania (Viannia) species.在由利什曼原虫（维安尼亚亚属）引起的皮肤利什曼病患者未受影响的黏膜组织中检测利什曼原虫。
J Infect Dis. 2009 Aug 15;200(4):638-46. doi: 10.1086/600109.
4
Evaluation of a microculture method for isolation of Leishmania parasites from cutaneous lesions of patients in Peru.秘鲁患者皮肤病变中利什曼原虫寄生虫分离的微培养方法评估
J Clin Microbiol. 2007 Nov;45(11):3680-4. doi: 10.1128/JCM.01286-07. Epub 2007 Sep 19.
5
Cutaneous leishmaniasis.皮肤利什曼病
Lancet Infect Dis. 2007 Sep;7(9):581-96. doi: 10.1016/S1473-3099(07)70209-8.
6
Isolation and molecular identification of Leishmania (Viannia) peruviana from naturally infected Lutzomyia peruensis (Diptera: Psychodidae) in the Peruvian Andes.从秘鲁安第斯山脉自然感染的秘鲁罗蛉（双翅目：毛蠓科）中分离和分子鉴定秘鲁利什曼原虫（维氏亚属）
Mem Inst Oswaldo Cruz. 2007 Aug;102(5):655-8. doi: 10.1590/s0074-02762007005000077.
7
Molecular diagnosis of leishmaniasis: current status and future applications.利什曼病的分子诊断：现状与未来应用
J Clin Microbiol. 2007 Jan;45(1):21-5. doi: 10.1128/JCM.02029-06. Epub 2006 Nov 8.
8
New developments in diagnosis of leishmaniasis.利什曼病诊断的新进展。
Indian J Med Res. 2006 Mar;123(3):311-30.
9
Comparison of PCR assays for diagnosis of cutaneous leishmaniasis.用于诊断皮肤利什曼病的聚合酶链反应检测方法比较
J Clin Microbiol. 2006 Apr;44(4):1435-9. doi: 10.1128/JCM.44.4.1435-1439.2006.
10
Development of a genetic assay to distinguish between Leishmania viannia species on the basis of isoenzyme differences.基于同工酶差异开发一种用于区分维氏利什曼原虫物种的基因检测方法。
Clin Infect Dis. 2006 Mar 15;42(6):801-9. doi: 10.1086/500326. Epub 2006 Feb 10.
Zotero 插件