Takase H, Minami M, Iwabuchi M
Department of Botany, Faculty of Science, Kyoto University, Japan.
Biochem Biophys Res Commun. 1991 May 15;176(3):1593-600. doi: 10.1016/0006-291x(91)90470-r.
We identified two novel DNA-binding proteins, ssDBP-1 and ssDBP-2, in wheat germ nuclear extract that interact with the proximal sequences of the promoter regions of the wheat histone H3 and H4 genes. Mobility shift and methylation interference assays have demonstrated that these factors specifically bind to the single-strand DNA which partially overlaps the hexamer and octamer cis-elements of the H3 promoter. Both proteins are distinguishable from HBP-1a and HBP-1b which specifically bind to the H3 hexamer sequence. These ssDNA-binding proteins are supposed to regulate the transcription of the wheat histone genes.
我们在小麦胚芽核提取物中鉴定出两种新型DNA结合蛋白，即ssDBP-1和ssDBP-2，它们与小麦组蛋白H3和H4基因启动子区域的近端序列相互作用。迁移率变动分析和甲基化干扰分析表明，这些因子特异性结合单链DNA，该单链DNA与H3启动子的六聚体和八聚体顺式元件部分重叠。这两种蛋白与特异性结合H3六聚体序列的HBP-1a和HBP-1b不同。这些单链DNA结合蛋白被认为可调节小麦组蛋白基因的转录。
