Mikami K, Sakamoto A, Takase H, Tabata T, Iwabuchi M
Department of Biology, Faculty of Science, Hokkaido University, Sapporo, Japan.
Nucleic Acids Res. 1989 Dec 11;17(23):9707-17. doi: 10.1093/nar/17.23.9707.
HBP-1 is a sequence-specific DNA-binding protein that interacts with the hexameric sequence ACGTCA, the putative cis-acting element of the wheat histone H3 gene. Gel mobility shift and DNase I footprint analyses showed that this protein interacts with homologous sequences in the regulatory regions for the transcription of the cauliflower mosaic virus (CaMV) 35S RNA and nopaline synthase (NOS) genes, evidence that HBP-1 may bind to hexameric sequences in the regulatory regions of various genes. An HBP-1-like protein, indistinguishable from wheat HBP-1 in its the DNA-binding specificity, is present in sunflower nuclear extract, an indication that HBP-1-like DNA-binding proteins also exist in dicots.
HBP-1是一种序列特异性DNA结合蛋白，它与六聚体序列ACGTCA相互作用，ACGTCA是小麦组蛋白H3基因假定的顺式作用元件。凝胶迁移率变动分析和DNase I足迹分析表明，该蛋白与花椰菜花叶病毒（CaMV）35S RNA和胭脂碱合酶（NOS）基因转录调控区域中的同源序列相互作用，这证明HBP-1可能与各种基因调控区域中的六聚体序列结合。在向日葵核提取物中存在一种与小麦HBP-1的DNA结合特异性无法区分的类HBP-1蛋白，这表明双子叶植物中也存在类HBP-1 DNA结合蛋白。
