Division of Endocrinology, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India.
Menopause. 2010 May-Jun;17(3):602-10. doi: 10.1097/gme.0b013e3181d0f7f0.
The aim of this study was to determine the skeletal effects of Butea total extract (BTE) and its acetone soluble fraction (ASF) from Butea monosperma, which is rich in methoxyisoflavones, in ovariectomized (OVx) rats, a model for postmenopausal bone loss.
BTE (1.0 g kg d) and ASF (100 mg kg d) were given orally for 12 weeks to adult OVx rats. The sham-operated and ovariectomy + vehicle groups served as controls. Bone mineral density, osteoid formation (mineral apposition rate and bone formation rate), bone microarchitecture, and bone turnover/resorption markers were studied. Phytoestrogens in rats given BTE and ASF were analyzed by high-performance liquid chromatography. One-way analysis of variance was used to test significance of effects.
OVx rats treated with either BTE or ASF exhibited increased bone mineral density in trabecular bones and improved trabecular microarchitecture compared with the ovariectomy + vehicle group. ASF treatment was more efficient than BTE treatment in maintaining trabecular microarchitecture. Serum osteocalcin and urinary type 1 collagen levels in OVx rats treated with either BTE or ASF were significantly lower than those of the ovariectomy + vehicle group. ASF treatment led to increased mineral apposition rate and bone formation rate compared with ovariectomy + vehicle, whereas BTE had no such effect. In the uterotropic assay, BTE was mildly estrogenic in adult OVx rats. In immature rats, BTE exhibited both estrogenicity and antiestrogenicity. ASF had neither uterine estrogenicity nor antiestrogenicity. Analysis of phytoestrogens revealed significant enrichment of cladrin, isoformononetin, and medicarpin in ASF over BTE.
Derived from B monosperma, ASF at a 10-fold lower dose than that of BTE was effective in preventing OVx-induced bone loss and stimulated new-bone formation.
本研究旨在确定富含甲氧基异黄酮的补骨脂总提取物（BTE）及其丙酮可溶性部分（ASF）对去卵巢（OVx）大鼠骨骼的影响，OVx 大鼠模型可用于研究绝经后骨质流失。
给成年 OVx 大鼠口服 BTE（1.0 g kg d）和 ASF（100 mg kg d）共 12 周。假手术和卵巢切除术+载体组作为对照。研究了骨矿物质密度、类骨质形成（矿化沉积率和骨形成率）、骨微结构以及骨转换/吸收标志物。采用高效液相色谱法分析给予 BTE 和 ASF 的大鼠中的植物雌激素。采用单因素方差分析检验效应的显著性。
与卵巢切除术+载体组相比，用 BTE 或 ASF 治疗的 OVx 大鼠的骨小梁骨矿物质密度增加，骨小梁微结构得到改善。ASF 治疗比 BTE 治疗更有效地维持骨小梁微结构。用 BTE 或 ASF 治疗的 OVx 大鼠的血清骨钙素和尿型胶原 I 水平明显低于卵巢切除术+载体组。与卵巢切除术+载体组相比，ASF 治疗导致矿化沉积率和骨形成率增加，而 BTE 则没有这种作用。在子宫增重试验中，BTE 在成年 OVx 大鼠中具有轻度雌激素作用。在未成熟大鼠中，BTE 表现出雌激素和抗雌激素作用。ASF 既没有子宫雌激素作用，也没有抗雌激素作用。植物雌激素分析显示，ASF 中 cladrin、isoformononetin 和 medicarpin 的含量明显高于 BTE。
源自 B monosperma 的 ASF，其剂量比 BTE 低 10 倍，能有效预防 OVx 引起的骨质流失并刺激新骨形成。
Zotero 插件