Division of Endocrinology, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India.
Menopause. 2010 Mar;17(2):393-402. doi: 10.1097/gme.0b013e3181bfae38.
This study aimed to determine the skeletal effects of total ethanolic extract (TEE) and its butanolic fraction (BF) from the stem-bark of Ulmus wallichiana, which is rich in C-glycosylated flavonoids, in growing rats (for peak bone [PB] achievement) and in ovariectomized (OVx) rats (for menopausal bone loss).
TEE (750 mg kg(-1) d(-1)) and BF (50 mg kg(-1) d(-1)) were given orally for 10 weeks to weaning female Sprague-Dawley rats and for 12 weeks to adult OVx rats of the same strain, respectively. In studies with OVx rats, sham operated + vehicle, OVx + 17beta-estradiol, and OVx + vehicle groups served as various controls. Bone mineral density (BMD), biomechanical strength, bone histology, formations of osteoprogenitor cells, osteoid formation, and bone turnover/resorption markers were studied. Bioactive marker compounds in TEE and BF were analyzed by high-performance liquid chromatography. One-way analysis of variance was used to test significance of effects.
In growing rats, both TEE and BF increased BMD, bone strength, and bone formation rate, suggesting higher PB achievement. OVx rats treated with either TEE or BF exhibited increased BMD at various anatomical positions and improved bone strength and trabecular architecture compared with the OVx + vehicle group. Serum osteocalcin and urinary type 1 collagen degradation product levels in OVx rats treated with either TEE or BF were significantly lower than those of the OVx + vehicle group. Neither TEE nor BF exhibited uterine estrogenicity. Analysis of marker compounds revealed significant enrichment of two bioactive markers in BF over TEE.
Derived from U wallichiana, BF at much a lower dose than TEE was effective in PB achievement and prevention of OVx-induced bone loss.
本研究旨在确定从富含 C-糖基化黄酮的榆属茎皮中提取的总乙醇提取物（TEE）及其丁醇部分（BF）对生长大鼠（达到峰值骨量[PB]）和去卵巢（OVx）大鼠（预防绝经后骨质流失）的骨骼影响。
分别给予 TE（750mg/kg/d）和 BF（50mg/kg/d）口服，持续 10 周给断奶雌性 Sprague-Dawley 大鼠，持续 12 周给同品系成年 OVx 大鼠。在 OVx 大鼠研究中，假手术+载体、OVx+17β-雌二醇和 OVx+载体组分别作为各种对照。研究了骨矿物质密度（BMD）、生物力学强度、骨组织学、成骨细胞形成、类骨质形成和骨转换/吸收标志物。采用高效液相色谱法分析 TEE 和 BF 中的生物活性标记化合物。采用单因素方差分析检验效应的显著性。
在生长大鼠中，TEE 和 BF 均增加了 BMD、骨强度和骨形成率，提示达到更高的 PB。与 OVx+载体组相比，用 TEE 或 BF 治疗的 OVx 大鼠在各个解剖部位的 BMD 增加，骨强度和小梁结构得到改善。用 TEE 或 BF 治疗的 OVx 大鼠的血清骨钙素和尿型 I 胶原降解产物水平明显低于 OVx+载体组。TEE 或 BF 均不具有子宫雌激素活性。标记化合物分析显示 BF 中两种生物活性标记物的含量明显高于 TEE。
源自榆属的 BF 在较低剂量下比 TEE 更有效，可达到 PB 并预防 OVx 引起的骨质流失。
