Magakian Yu A, Karalyan Z A, Karalova E M, Abroyan L O, Akopyan L A, Gasparyan M H, Jaghacpanyan N G, Semerjyan Z B, Ter-Pogossyan Z R
Institute of Molecular Biology, National Academy of Sciences of the Republic of Armenia, Erevan, Armenia.
Bull Exp Biol Med. 2009 Oct;148(4):615-8. doi: 10.1007/s10517-010-0778-6.
Reactions of continuous HeLa and RD cell cultures and their nuclear and nucleolar apparatus to addition of solcoseryl into the medium were studied. The monolayer density, proliferation activity, percentage of dead cells, RNA and DNA content in the nuclei and nucleoli, number of nucleoli in the nuclei, cell distribution in the population by the number of nucleoli in the nuclei, volume and complete surface area of the nuclei and nucleoli, and the nucleolar/nuclear ratio were evaluated. The cultures differently reacted to solcoseryl in the medium at the population and cellular levels of their organization. By the results of multiparametric analysis of the reactions of cells and their nuclear and nucleolar apparatus, solcoseryl can be referred to bioactive substances with characteristics of a factor regulating cell population growth.
研究了连续培养的HeLa细胞和RD细胞及其细胞核和核仁装置对向培养基中添加舒康博的反应。评估了单层密度、增殖活性、死细胞百分比、细胞核和核仁中的RNA和DNA含量、细胞核中的核仁数量、细胞核中核仁数量在细胞群体中的分布、细胞核和核仁的体积和完整表面积以及核仁/核比率。这些培养物在其组织的群体和细胞水平上对培养基中的舒康博有不同反应。根据对细胞及其细胞核和核仁装置反应的多参数分析结果，舒康博可被归类为具有调节细胞群体生长因子特征的生物活性物质。
