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[舒血管素对海马组织外植体培养的影响]

[The effect of Solcoseryl on explant cultures of the hippocampus].

作者信息

Lindner G, Grosse G, Goworek K, Franz C, Liebezeit K

出版信息

Z Mikrosk Anat Forsch. 1979;93(2):305-14.

PMID:532265
Abstract

Explants of hippocampus from fetal rats were cultivated in Maximow chambers in semisynthetic medium up to 12 days in vitro. The cultures were fixed Bouin, slided 15 micron, coloured with Klüver-Barrera and some morphological parameters were tested. 1. The nerve fiber index increased by influence of 1% Solcoseryl in relation to control cultures, which growed in minimal medium. An essential stimulation was observed by application of placentar serum and embryonal extract into the culture medium. 2. Die decrease of the number of neurons and glial cells per unit of area and a small decrease of the area of neuron nuclei was discussed in relation to the effect of the pharmacon Solcoseryl on O2- consumption. 3. Solcoseryl (firm Solco AG, Base) is an extract of calf blood. It can not substitute other tissue extracts.

摘要

将胎鼠海马体的外植体置于马克西莫培养室,在半合成培养基中培养至体外12天。培养物用布因氏液固定,切成15微米厚的切片,用克吕弗-巴雷拉染色法染色,并检测一些形态学参数。1. 与在基础培养基中生长的对照培养物相比,1%的血活素能使神经纤维指数增加。向培养基中添加胎盘血清和胚胎提取物时,可观察到显著的刺激作用。2. 讨论了药物血活素对氧消耗的影响与单位面积神经元和神经胶质细胞数量减少以及神经元细胞核面积略有减小之间的关系。3. 血活素(索尔科AG公司,巴塞尔)是小牛血液的提取物。它不能替代其他组织提取物。

相似文献

1
[The effect of Solcoseryl on explant cultures of the hippocampus].[舒血管素对海马组织外植体培养的影响]
Z Mikrosk Anat Forsch. 1979;93(2):305-14.
2
[Effect of Solcoseryl on nerve tissue under in vitro conditions].[舒可捷(索高捷疗)在体外条件下对神经组织的作用]
Z Mikrosk Anat Forsch. 1976;90(3):416-26.
3
[The effect of Solcoseryl on in-vitro cultured cells].[舒康博对体外培养细胞的作用]
Z Mikrosk Anat Forsch. 1977;91(2):195-214.
4
[The effect of orotic acid and sodium orotate on organ cultures of the hippocampus of embryonic rats].
Z Mikrosk Anat Forsch. 1980;94(2):283-91.
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[Effect of the culture technic on the development of the hippocampus (rat) in vitro].[培养技术对体外海马体(大鼠)发育的影响]
Z Mikrosk Anat Forsch. 1982;96(3):527-36.
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[The effect of a synthetic tripeptide nervous tissue cultured in vitro].
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[Effect of substance P on nerve fiber regeneration in tissue culture].[P物质对组织培养中神经纤维再生的影响]
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[The development of nervous tissue to experimental manipulation in vitro (author's transl)].[神经组织在体外对实验操作的反应(作者译)]
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The effects of solcoseryl on the growth and multiplication of chick embryo fibroblasts cultivated "in vitro".舒康博对体外培养的鸡胚成纤维细胞生长及增殖的影响。
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