Ramsden C S, Drayson M T, Bell E B
Department of Cell and Structural Biology Medical School, University of Manchester, UK.
Br J Cancer. 1991 May;63(5):699-704. doi: 10.1038/bjc.1991.158.
Lymphocytes were studied as vehicles to target the plant toxin ricin, to lymphoid tissue in rats. Ricin-loaded thoracic duct lymphocyte (TDL) migrated normally into lymph nodes (LN) at 0.5 h, but this process was arrested by 3 h after injection. Ricin was successfully targeted to lymphoid tissue as evidenced by a 4-fold increase in ricin-associated radioactivity in LN, a 10-fold increase in the Peyers patches, a doubling in the spleen and a 35% reduction of radioactivity in the liver compared with free ricin. Nevertheless this represented a considerable shortfall in the expected targeting efficiency. The main problem was found to be high in vivo elution of ricin from TDL (70% within 0.5 h of i.v injection). This and other aspects relevant to maximising targeting efficiency are discussed.
淋巴细胞作为将植物毒素蓖麻毒素靶向大鼠淋巴组织的载体进行了研究。负载蓖麻毒素的胸导管淋巴细胞(TDL)在0.5小时时正常迁移至淋巴结(LN),但注射后3小时此过程被阻断。与游离蓖麻毒素相比,LN中蓖麻毒素相关放射性增加4倍、派伊尔结增加10倍、脾脏中增加一倍以及肝脏中放射性降低35%,证明蓖麻毒素成功靶向至淋巴组织。然而,这在预期的靶向效率方面仍存在相当大的不足。主要问题是TDL中蓖麻毒素在体内的高洗脱率(静脉注射后0.5小时内达70%)。本文讨论了这一点以及与最大化靶向效率相关的其他方面。