CTCPA Centre Technique de la Conservation des Produits Agricoles, Site Agroparc, ZA de l'aéroport, BP 21 203, 84911, AVIGNON cedex 9, France.
Curr Microbiol. 2010 Dec;61(6):525-33. doi: 10.1007/s00284-010-9648-8. Epub 2010 Apr 16.
Thermophilic bacteria that form highly heat-resistant spores constitute an important group of spoilage bacteria of low-acid canned food. A PCR assay was developed in order to rapidly trace these bacteria. Three PCR primer pairs were designed from rRNA gene sequences. These primers were evaluated for the specificity and the sensitivity of detection. Two primer pairs allowed detection at the species level of Geobacillus stearothermophilus and Moorella thermoacetica/thermoautrophica. The other pair allowed group-specific detection of anaerobic thermophilic bacteria of the genera Thermoanaerobacterium, Thermoanaerobacter, Caldanerobium and Caldanaerobacter. After a single enrichment step, these PCR assays allowed the detection of 28 thermophiles from 34 cans of spoiled low-acid food. In addition, 13 ingredients were screened for the presence of these bacteria. This PCR assay serves as a detection method for strains able to spoil low-acid canned food treated at 55°C. It will lead to better reactivity in the canning industry. Raw materials and ingredients might be qualified not only for quantitative spore contamination, but also for qualitative contamination by highly heat-resistant spores.
能够形成高度耐热孢子的嗜热细菌是低酸性罐头食品中重要的腐败菌群体。为了快速追踪这些细菌，我们开发了一种 PCR 检测方法。我们从 rRNA 基因序列中设计了 3 对 PCR 引物。评估了这些引物的特异性和检测灵敏度。其中两对引物可以在种水平上检测到嗜热脂肪地芽孢杆菌和嗜热醋菌/嗜热自养菌。另一对引物可以特异性检测厌氧嗜热细菌属 Thermoanaerobacterium、Thermoanaerobacter、Caldanerobium 和 Caldanaerobacter。经过单一的富集步骤，这些 PCR 检测方法可以从 34 罐变质的低酸性食品中检测到 28 种嗜热菌。此外，还筛选了 13 种成分中是否存在这些细菌。该 PCR 检测方法可用于检测能够在 55°C 处理的低酸性罐装食品中腐败的菌株。这将提高罐头工业的反应能力。原材料和成分不仅可以定量检测孢子污染，还可以定性检测高度耐热孢子的污染。
