First Department of Pathologic Anatomy, Medical Faculty of Masaryk University and St. Anne's University Hospital, Pekarska 53, 65691 Brno, Czech Republic.
World J Gastroenterol. 2010 Apr 21;16(15):1879-84. doi: 10.3748/wjg.v16.i15.1879.
To perform a comparative analysis of clinicopathological correlations of cyclooxygenase-2 (COX-2) expression in pancreatic cancer, examined by monoclonal and polyclonal antibodies.
The COX-2 expression in 85 resection specimens of pancreatic ductal adenocarcinoma was immunohistochemically examined using both monoclonal and polyclonal antibodies. The final immunoscores were obtained by multiplying the percentage of positive cells with the numeric score reflecting the staining intensity. COX-2 expression levels were classified into three categories (0, 1+, and 2+) and the clinicopathological correlations were statistically evaluated and analyzed.
The positive tumor expression rates of COX-2 were 80.5% using monoclonal antibody and 69.4% using polyclonal antibody. In the Kaplan-Meier analysis, no significant correlations were found between levels of COX-2 expression and overall survival (OS), but trends to longer OS were found in COX-2 negative cases using monoclonal antibody. Significantly longer disease free survival was revealed in COX-2 negative cases using monoclonal antibody (P = 0.019). No correlations between COX-2 expression levels and grade (G), tumor (T) status and nodal (N) status were demonstrated. Low histological grade showed a strong association with a longer OS (P < 0.001). Correlation of survival and T status revealed a shorter OS in T3 tumors, but the results reached only marginal statistical significance (P = 0.070). In the multivariate Cox proportional hazards regression model, histological grade, T and N status remained valuable predictors of a worse survival with borderline significance for T [hazards ratio (HR) = 4.18 for G (if G = 3, P < 0.001); HR = 1.64 for T (if T = 3, P = 0.065); HR = 2.53 for N (if N = 1, P = 0.006)]. Higher grade, T or N status was associated with a worse OS.
The immunohistochemically assessed level of COX-2 expression does not seem to represent a valuable independent prognostic factor and is not superior to the conventional prognostic factors.
通过使用单克隆和多克隆抗体，对 COX-2 在胰腺癌中的表达进行临床病理相关性的比较分析。
采用免疫组织化学方法，对 85 例胰腺导管腺癌的 COX-2 表达进行了检测，使用了单克隆和多克隆抗体。最终的免疫评分通过将阳性细胞的百分比与反映染色强度的数值评分相乘获得。COX-2 表达水平分为 3 个等级（0、1+和 2+），然后对临床病理相关性进行了统计学评估和分析。
使用单克隆抗体时，COX-2 的肿瘤阳性表达率为 80.5%，使用多克隆抗体时为 69.4%。在 Kaplan-Meier 分析中，COX-2 表达水平与总生存（OS）之间没有显著相关性，但使用单克隆抗体的 COX-2 阴性病例有延长 OS 的趋势。使用单克隆抗体的 COX-2 阴性病例无病生存期显著延长（P=0.019）。COX-2 表达水平与分级（G）、肿瘤（T）状态和淋巴结（N）状态之间没有相关性。低组织学分级与更长的 OS 有很强的相关性（P<0.001）。生存与 T 状态的相关性显示 T3 肿瘤的 OS 较短，但结果仅达到边缘统计学意义（P=0.070）。在多变量 Cox 比例风险回归模型中，组织学分级、T 和 N 状态仍然是生存较差的有价值的预测因子，T 状态具有边缘统计学意义（HR=4.18，G=3，P<0.001；HR=1.64，T=3，P=0.065；HR=2.53，N=1，P=0.006）。更高的分级、T 或 N 状态与更差的 OS 相关。
免疫组化评估的 COX-2 表达水平似乎不是一个有价值的独立预后因素，也不比传统的预后因素优越。
