双抗体夹心酶联免疫吸附试验检测软壳龟虹彩病毒抗原。
A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens.
机构信息
The Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, No. 241 Building, Huixinxijie, Chaoyang District, Beijing 100029, China.
出版信息
J Virol Methods. 2010 Aug;167(2):193-8. doi: 10.1016/j.jviromet.2010.04.004. Epub 2010 Apr 23.
A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle iridovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum. Using DAS-ELISA, the detection limit of STIV was found to be 10(3)PFU/ml. The positive rate of 15 STIV samples was 100%, while the positive rate of 100 other aquatic virus samples was 0%. These data show that DAS-ELISA is highly specific and sensitive for the detection of STIV. In clinical tests, 128 samples isolated from pond-reared turtles were subjected to DAS-ELISA and PCR. The overall agreement between the results obtained by DAS-ELISA and PCR was 98.4%. The results indicate that the DAS-ELISA method could be used for diagnosing diseases caused by STIV.
建立了一种检测中华鳖疱疹病毒（STIV）的双抗体夹心酶联免疫吸附试验（DAS-ELISA），该方法使用针对 STIV 的特异性单克隆抗体（mAb）和抗 STIV 兔血清。使用 DAS-ELISA，发现 STIV 的检测限为 10(3)PFU/ml。15 份 STIV 样本的阳性率为 100%，而 100 份其他水生病毒样本的阳性率为 0%。这些数据表明，DAS-ELISA 对 STIV 的检测具有高度特异性和敏感性。在临床检测中，对来自池塘养殖鳖的 128 个样本进行了 DAS-ELISA 和 PCR 检测。DAS-ELISA 和 PCR 检测结果的总符合率为 98.4%。结果表明，DAS-ELISA 方法可用于诊断由 STIV 引起的疾病。
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