用于检测人血清中严重急性呼吸综合征相关冠状病毒抗体的双抗原夹心酶联免疫吸附测定法
Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum.
作者信息
Chen S, Lu D, Zhang M, Che J, Yin Z, Zhang S, Zhang W, Bo X, Ding Y, Wang S
机构信息
Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, People's Republic of China.
出版信息
Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):549-53. doi: 10.1007/s10096-005-1378-7.
Abstract
The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.
摘要
开展本研究是为了评估双抗原夹心酶联免疫吸附测定法（ELISA）检测人血清中针对严重急性呼吸综合征（SARS）相关冠状病毒抗体的性能。SARS相关冠状病毒的重组部分核衣壳蛋白用作ELISA中的血清诊断抗原。使用ELISA试剂盒检测了总共2892份临床血清样本，该试剂盒阳性鉴定出35例确诊SARS感染患者中的25例（71.4%）、407例疑似SARS患者中的286例（70%）、302例SARS康复患者中的229例（75.8%）以及544份医护人员样本中的0例；在1604份来自其他疾病患者的临床样本中，只有1例呈弱阳性结果。这些结果表明，双抗原夹心ELISA是SARS相关冠状病毒血清诊断的一种有效筛查方法。
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