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本文引用的文献

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Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.基于单克隆抗体的灵敏且特异的捕获酶免疫测定法，用于检测严重急性呼吸综合征患者血清中的核衣壳抗原。
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Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia.检测针对严重急性呼吸综合征（SARS）冠状病毒核衣壳蛋白的特异性抗体用于SARS冠状病毒肺炎的血清学诊断。
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Profiles of antibody responses against severe acute respiratory syndrome coronavirus recombinant proteins and their potential use as diagnostic markers.针对严重急性呼吸综合征冠状病毒重组蛋白的抗体反应谱及其作为诊断标志物的潜在用途。
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Recombinant protein-based enzyme-linked immunosorbent assay and immunochromatographic tests for detection of immunoglobulin G antibodies to severe acute respiratory syndrome (SARS) coronavirus in SARS patients.基于重组蛋白的酶联免疫吸附测定和免疫层析试验用于检测严重急性呼吸综合征（SARS）患者中针对SARS冠状病毒的免疫球蛋白G抗体。
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Genetic evidence for a structural interaction between the carboxy termini of the membrane and nucleocapsid proteins of mouse hepatitis virus.小鼠肝炎病毒膜蛋白和核衣壳蛋白羧基末端之间存在结构相互作用的遗传学证据。
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用于检测人血清中严重急性呼吸综合征相关冠状病毒抗体的双抗原夹心酶联免疫吸附测定法

Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum.

作者信息

Chen S, Lu D, Zhang M, Che J, Yin Z, Zhang S, Zhang W, Bo X, Ding Y, Wang S

机构信息

Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, People's Republic of China.

出版信息

Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):549-53. doi: 10.1007/s10096-005-1378-7.

DOI:10.1007/s10096-005-1378-7
PMID:16133409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7088218/
Abstract

The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.

摘要

开展本研究是为了评估双抗原夹心酶联免疫吸附测定法（ELISA）检测人血清中针对严重急性呼吸综合征（SARS）相关冠状病毒抗体的性能。SARS相关冠状病毒的重组部分核衣壳蛋白用作ELISA中的血清诊断抗原。使用ELISA试剂盒检测了总共2892份临床血清样本，该试剂盒阳性鉴定出35例确诊SARS感染患者中的25例（71.4%）、407例疑似SARS患者中的286例（70%）、302例SARS康复患者中的229例（75.8%）以及544份医护人员样本中的0例；在1604份来自其他疾病患者的临床样本中，只有1例呈弱阳性结果。这些结果表明，双抗原夹心ELISA是SARS相关冠状病毒血清诊断的一种有效筛查方法。