Department of Soil Science, College of Agriculture and Life Science, North Carolina State University, Campus Box 7619, Raleigh, NC 27695, USA.
Res Microbiol. 2010 Jun;161(5):315-25. doi: 10.1016/j.resmic.2010.03.010. Epub 2010 Apr 29.
Soil-denitrifying bacteria in highly-managed turfgrass systems were examined to assess their response to land-use change and time under management. Denitrifier community composition and diversity in a turfgrass chronosequence of 1 to 95-years-old were compared with those in an adjacent pine-dominant forest via molecular investigations of nirK and nosZ gene fragments. Both denaturing gradient gel electrophoresis and sequenced clone libraries revealed that the denitrifier community became more diverse after turf establishment, and the diversity was then preserved. Furthermore, the composition of the turfgrass denitrifier community was slightly affected by time under management. Meta-analysis of sequenced nirK and nosZ gene fragments from a variety of ecosystems showed that denitrifier communities in pine and turf were more similar to those in other environments than to each other, suggesting that land-use change substantially modified the composition and increased the diversity of denitrifiers. This study provides a useful baseline of nirK- and nosZ-type soil denitrifier communities to aid in the evaluation of ecological and environmental impacts of turfgrass systems.
本研究通过对土壤中nirK 和 nosZ 基因片段的分子研究，调查了高度管理的草坪系统中的土壤脱氮细菌，以评估它们对土地利用变化和管理时间的响应。通过与相邻的以松树为主的森林进行比较，研究了 1 至 95 年生草坪演替系列中脱氮菌群落的组成和多样性。变性梯度凝胶电泳和测序克隆文库的结果均表明，草坪建立后脱氮菌群落的多样性增加，并且多样性得到了保持。此外，草坪脱氮菌群落的组成受管理时间的轻微影响。对来自各种生态系统的测序 nirK 和 nosZ 基因片段的荟萃分析表明，松树和草坪中的脱氮菌群落与其他环境中的脱氮菌群落更为相似，而彼此之间的相似性较低，这表明土地利用变化极大地改变了脱氮菌的组成并增加了其多样性。本研究为 nirK 和 nosZ 型土壤脱氮菌群落提供了有用的基线，有助于评估草坪系统对生态和环境的影响。
