Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, Marburg, Germany.
FEMS Microbiol Ecol. 2010 Jul 1;73(1):134-48. doi: 10.1111/j.1574-6941.2010.00884.x. Epub 2010 Apr 8.
The impacts of temperature on the activity and on the size as well as on the community composition of denitrifiers in an agricultural soil were studied in a controlled laboratory experiment. Soil slurries were incubated at different temperatures (4, 15, 20, 25, and 37 degrees C) under nonlimiting substrate conditions for 3 weeks. The abundance of the nitrate-reducer community in general was determined using the most probable number (MPN) technique; denitrifier activity and community composition were assessed by measuring potential denitrifier enzyme activity and by terminal restriction fragment length polymorphisms as well as by phylogenetic analysis of nitrite reductase gene amplicons (nirK and nirS). Increasing incubation temperatures resulted in gradually enhanced denitrification activity, but also in higher abundance of nitrate reducers and in different denitrifier community compositions. Genetic and physiological characterization of isolates purified from the highest dilution of the MPN series emphasized community differences. Overall, temperature apparently not only affected process rates but also resulted in the enrichment of denitrifiers and shifts in the community composition.
在一项控制实验室实验中，研究了温度对农业土壤中反硝化菌的活性、大小以及群落组成的影响。在非限制底物条件下，将土壤泥浆在不同温度（4、15、20、25 和 37°C）下孵育 3 周。使用最可能数（MPN）技术确定硝酸盐还原菌群落的丰度；通过测量潜在的反硝化酶活性以及末端限制性片段长度多态性以及通过亚硝酸盐还原酶基因扩增子（nirK 和 nirS）的系统发育分析来评估反硝化菌活性和群落组成。随着培养温度的升高，反硝化作用逐渐增强，但硝酸盐还原菌的丰度也更高，反硝化菌群落组成也不同。从 MPN 系列最高稀释度分离出的纯化物的遗传和生理特性强调了群落差异。总的来说，温度不仅明显影响了过程速率，而且导致了反硝化菌的富集和群落组成的变化。
