Center for Nanomedicine Research, National Health Research Institutes, Zhunan 350, Taiwan.
Nitric Oxide. 2010 Aug 1;23(1):60-4. doi: 10.1016/j.niox.2010.04.003. Epub 2010 Apr 22.
Zwitterionic diazeniumdiolate, a nitric oxide precursor, was dissolved in basic buffer solution (pH=9.0) and encapsulated in thermo-sensitive liposomes composed of phospholipids of different sensitive temperatures. The basic intra-liposomal environment dramatically delayed spontaneous NO release. When the liposomes were placed in physiological buffer solution and temperatures were increased to the sensitive temperatures of the phospholipids' membranes, extra-liposomal protons started to influx to collapse the pH gradient and subsequently induce a significant NO release. Moreover, the presence of a stronger pH gradient when the liposomes were applied to a more acidic environment caused a higher proton influx driving force to trigger the influx of protons and, subsequently, NO release. In this work, we demonstrate that thermo-sensitive liposomes can be used to create a stable pH gradient in a nanoenvironment entrapping zwitterionic diazeniumdiolate for sustained and heat/acid-activated NO release.
两性离子二氮烯二酸盐，一种一氧化氮前体，溶解在碱性缓冲溶液（pH=9.0）中，并封装在由不同敏感温度的磷脂组成的温敏脂质体中。碱性的腔内环境极大地延迟了自发的一氧化氮释放。当脂质体被置于生理缓冲溶液中且温度升高到磷脂膜的敏感温度时，额外的脂质体质子开始内流以破坏 pH 梯度，从而随后诱导显著的一氧化氮释放。此外，当脂质体应用于更酸性的环境时，存在更强的 pH 梯度会导致更高的质子内流驱动力，以触发质子的内流，从而随后释放一氧化氮。在这项工作中，我们证明了温敏脂质体可用于在纳米环境中产生稳定的 pH 梯度，从而将两性离子二氮烯二酸盐包封起来，以实现持续的、热/酸激活的一氧化氮释放。
