Bouma H R, Strijkstra A M, Boerema A S, Deelman L E, Epema A H, Hut R A, Kroese F G M, Henning R H
Department of Clinical Pharmacology, Groningen University Institute for Drug Exploration (GUIDE), University Medical Center Groningen, The Netherlands.
Vet Immunol Immunopathol. 2010 Aug 15;136(3-4):319-23. doi: 10.1016/j.vetimm.2010.03.016. Epub 2010 Mar 30.
Hibernation is a unique natural model to study large and specific modulation in numbers of leukocytes and thrombocytes, with potential relevance for medical application. Hibernating animals cycle through cold (torpor) and warm (arousal) phases. Previous research demonstrated clearance of leukocytes and thrombocytes from the circulation during torpor, but did not provide information regarding the timing during torpor or the subtype of leukocytes affected. To study the influence of torpor-bout duration on clearance of circulating cells, we measured blood cell dynamics in the European Ground Squirrel. Numbers of leukocytes and thrombocytes decreased within 24h of torpor by 90% and remained unchanged during the remainder of the torpor-bout. Differential counts demonstrated that granulocytes, lymphocytes and monocytes are all affected by torpor. Although a decreased production might explain the reduced number of thrombocytes, granulocytes and monocytes, this cannot explain the observed lymphopenia since lymphocytes have a much lower turnover rate than thrombocytes, granulocytes and monocytes. In conclusion, although underlying biochemical signaling pathways need to be unraveled, our data show that the leukocyte count drops dramatically after entrance into torpor and that euthermic cell counts are restored within 1.5h after onset of arousal, even before body temperature is fully normalized.
冬眠是一种独特的自然模型，可用于研究白细胞和血小板数量的大规模且特定的调节，这对医学应用具有潜在意义。冬眠动物会经历寒冷（蛰伏）和温暖（觉醒）阶段循环。先前的研究表明，在蛰伏期间白细胞和血小板会从循环中清除，但未提供关于蛰伏期间的时间或受影响白细胞亚型的信息。为了研究蛰伏期持续时间对循环细胞清除的影响，我们测量了欧洲地松鼠的血细胞动态。在蛰伏开始后的24小时内，白细胞和血小板数量减少了90%，在蛰伏期的其余时间内保持不变。分类计数表明，粒细胞、淋巴细胞和单核细胞均受蛰伏影响。虽然产量降低可能解释了血小板、粒细胞和单核细胞数量的减少，但这无法解释观察到的淋巴细胞减少，因为淋巴细胞的周转率比血小板、粒细胞和单核细胞低得多。总之，虽然潜在的生化信号通路有待阐明，但我们的数据表明，进入蛰伏后白细胞计数会急剧下降，并且在觉醒开始后1.5小时内，即使体温尚未完全恢复正常，正常体温下的细胞计数也会恢复。
