Clinic of Child Neuropsychiatry, Second University of Naples, Via Pansini 5, Naples, Italy.
Seizure. 2010 Jun;19(5):296-9. doi: 10.1016/j.seizure.2010.03.004.
Despite anticonvulsant efficacy in animal models of generalized epilepsy, levetiracetam was not effective in the maximal subcutaneous PTZ model in mice and rats. Aim of this study was to assess the efficacy of levetiracetam (LEV) against submaximal, s.c. MET test (PTZ at the dose of 70 mg/kg) acute seizures in Wistar rats, in comparison to valproic acid (VPA). Thirty male Wistar rats (P42) were divided in three drug-treatment groups (10 rats in each group) as follows: valproic acid, levetiracetam, and controls. All animals were tested for seizure threshold at age P50. VPA (110 mg/kg) and LEV (108 mg/kg) were freshly dissolved in saline and injected i.p. in 2-3 ml/kg, 15 and 30 min, respectively, before pentylenetetrazol (PTZ) injection at the dose of 70 mg/kg. The average latency of the seizure type 3 (generalized clonic seizure with loss of righting reflexes) significantly differed between controls and the drug-treated animal groups (p < or = 0.02). The average duration of the seizure type 2 (threshold seizure) was significantly longer in both groups compared to controls (<0.02). In conclusion, LEV plays a role against seizures triggered by subcutaneous PTZ injection given at submaximal doses in rats, as demonstrated by a significant increase in duration of the seizure type 2 (threshold seizure).
尽管在全面性癫痫的动物模型中具有抗惊厥作用，但左乙拉西坦在小鼠和大鼠的最大皮下 PTZ 模型中无效。本研究的目的是评估左乙拉西坦 (LEV) 对亚最大剂量、皮下 MET 测试（70mg/kgPTZ）急性发作的有效性，与丙戊酸（VPA）进行比较。30 只雄性 Wistar 大鼠（P42）分为三组药物治疗组（每组 10 只大鼠）：丙戊酸、左乙拉西坦和对照组。所有动物在 P50 年龄时均进行了癫痫发作阈值测试。VPA（110mg/kg）和 LEV（108mg/kg）分别在戊四氮（PTZ）注射前 15 分钟和 30 分钟，以 2-3ml/kg 的剂量新鲜溶解在生理盐水中并腹腔注射。PTZ 以 70mg/kg 的剂量注射。第 3 型（全身性强直阵挛性癫痫伴失去翻正反射）的平均发作潜伏期在对照组和药物治疗动物组之间有显著差异（p <或= 0.02）。与对照组相比，第 2 型（阈值发作）的平均发作持续时间在两组中均显著延长（<0.02）。总之，LEV 在大鼠中发挥作用，可对抗亚最大剂量皮下 PTZ 注射引发的发作，这表现为第 2 型（阈值发作）的发作持续时间显著延长。
