RWTH Aachen University, Institute of Biology VII, Molecular Biotechnology, Worringerweg 1, 52074 Aachen, Germany.
Biosens Bioelectron. 2011 Jan 15;26(5):1895-901. doi: 10.1016/j.bios.2010.03.026. Epub 2010 Mar 25.
The importance of early diagnosis devices increased continuously in the last two decades and plays an important role in medical care. Early stage diagnosis of e.g. ovarian cancer, HCV-infection or HIV-infection increased the survival rate of patients significantly. In parallel there is a trend leaving centralized diagnostic laboratories in order to get closer to the patient to perform analysis of even complex parameters in the field. This often saves time, increases the prognosis of the patient significantly and is cheaper in many cases. In this study we employ a rapid and cost-effective detection system based on electrical biochip technology for decentralized detection of anti-HCV Core immunoglobulins (HCV antibodies). In this system the qualitative and quantitative detection of virus-specific antibodies is done by an ELISA directly on a gold electrode array utilizing HCV Core as capture antigen. The biochip allows antibody detection within 20 min. Signal amplification was done by enzyme labelling and by "Single Electrode Redox Cycling". This method enhances current signals up to 40-fold in comparison to simple oxidation. The sensitivity of this approach is therefore comparable to a standard microtiter plate based ELISA with a 9-fold saving of assay time. This biochip system allows serum or whole blood analysis with no signal loss or increasing background caused by the red blood cells. Fields of application can be hospital emergency units where only single detections have to be conducted in a quick manner or by the general practitioner.
在过去的二十年中，早期诊断设备的重要性不断增加，在医疗保健中发挥着重要作用。例如，卵巢癌、丙型肝炎病毒感染或艾滋病病毒感染的早期诊断显著提高了患者的生存率。与此同时，有一种趋势是离开集中的诊断实验室，以便更接近患者，在现场分析甚至复杂的参数。这在许多情况下通常可以节省时间，显著提高患者的预后，并降低成本。在这项研究中，我们采用了一种快速且具有成本效益的基于电生物芯片技术的检测系统，用于分散检测抗 HCV 核心免疫球蛋白（HCV 抗体）。在该系统中，通过 HCV 核心作为捕获抗原，在金电极阵列上直接进行 ELISA 定性和定量检测，可检测病毒特异性抗体。生物芯片可在 20 分钟内完成抗体检测。通过酶标记和“单电极氧化还原循环”进行信号放大。与简单的氧化相比，这种方法将电流信号增强了 40 倍。因此，该方法的灵敏度与基于标准微量滴定板的 ELISA 相当，检测时间节省了 9 倍。该生物芯片系统允许对血清或全血进行分析，不会因红细胞而导致信号丢失或背景增加。应用领域可以是医院急诊部门，那里只需快速进行单次检测，也可以是全科医生。
