Department of Psychobiology, Universidade Federal de São Paulo (UNIFESP), Brazil.
Eur J Pain. 2010 Nov;14(10):985-91. doi: 10.1016/j.ejpain.2010.03.008. Epub 2010 Apr 18.
This study aims to evaluate the impact of neonatal arthritis on adult pain threshold, sleep and general behaviours in rats and their lactating dams. Male pups were injected in the hind paw with complete Freund's adjuvant or saline on postnatal day (PN) 1. After weaning, dams were tested for anxiety, sleep recording or hormone profiling (ACTH, corticosterone and prolactin) and brain sampling (pineal melatonin and hippocampus serotonin). At adulthood (PN90), distinct subgroups of neonatal arthritic (AR) and control rats (CR) were also assessed for anxiety and pain thresholds, sleep recording, and blood/brain sampling. Compared to their respective controls at PN12, dams of arthritic rats (DAR) showed a longer latency in expressing pup retrieval and dam-pup interaction. DAR and AR showed a lower pain threshold, anxiety-like behaviour, and sleep fragmentation. Compared to controls, DAR displayed longer sleep latency, reduced paradoxical sleep latency and sleep efficiency, a decrease in prolactin and serotonin levels and increased melatonin levels. This model of unilateral hindpaw inflammation has a wide range of long-term effects in both lactating dams and their adult offspring.
本研究旨在评估新生儿关节炎对大鼠及其哺乳期母鼠成年后疼痛阈值、睡眠和一般行为的影响。雄性幼鼠于出生后第 1 天（PN）在后爪注射完全弗氏佐剂或生理盐水。断乳后，对母鼠进行焦虑、睡眠记录或激素分析（ACTH、皮质酮和催乳素）和脑取样（松果体褪黑素和海马体 5-羟色胺）。在成年期（PN90），还对不同亚组的新生儿关节炎（AR）和对照大鼠（CR）进行了焦虑和疼痛阈值、睡眠记录以及血液/脑组织取样评估。与 PN12 时各自的对照相比，关节炎大鼠的母鼠（DAR）在表达幼鼠取回和母鼠-幼鼠互动方面表现出更长的潜伏期。DAR 和 AR 表现出较低的疼痛阈值、焦虑样行为和睡眠碎片化。与对照组相比，DAR 显示出更长的睡眠潜伏期、降低的异相睡眠潜伏期和睡眠效率、催乳素和 5-羟色胺水平降低以及褪黑素水平升高。这种单侧后爪炎症模型对哺乳期母鼠及其成年后代都有广泛的长期影响。
