利用 DNA 免疫技术制备抗裂谷热病毒核衣壳蛋白单克隆抗体的研制与鉴定。
Development and characterization of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein generated by DNA immunization.
机构信息
Centro de Investigación en Sanidad Animal, Carretera de Valdeolmos, Valdeolmos, Madrid, Spain.
出版信息
MAbs. 2010 May-Jun;2(3):275-84. doi: 10.4161/mabs.2.3.11676. Epub 2010 May 2.
Abstract
This paper describes the generation of monoclonal antibodies directed to immunogenic nucleoprotein N epitopes of Rift Valley fever virus (RVFV), and their application in diagnostics, both for antibody detection in competitive ELISA and for antigen capture in a sandwich ELISA. Monoclonal antibodies (mAbs) were generated after DNA immunization of Balb/c mice and characterized by western blot, ELISA and cell immunostaining assays. At least three different immunorelevant epitopes were defined by mAb competition assays. Interestingly, two of the mAbs generated were able to distinguish between RVFV strains from Egyptian or South African lineages. These monoclonal antibodies constitute useful tools for diagnosis, especially for the detection of serum anti-RVFV antibodies from a broad range of species by means of competitive ELISA.
摘要
本文描述了针对裂谷热病毒（RVFV）免疫原性核蛋白 N 表位的单克隆抗体的产生，以及它们在诊断中的应用，包括竞争 ELISA 中的抗体检测和夹心 ELISA 中的抗原捕获。通过 DNA 免疫 Balb/c 小鼠产生了单克隆抗体，并通过 Western blot、ELISA 和细胞免疫染色分析进行了表征。通过 mAb 竞争分析确定了至少三个不同的免疫相关表位。有趣的是，产生的两种 mAb 能够区分来自埃及或南非谱系的 RVFV 株。这些单克隆抗体构成了有用的诊断工具，特别是通过竞争 ELISA 检测来自广泛物种的血清抗 RVFV 抗体。
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