Novo F J, Tutor J C
Laboratorio Central, Hospital General de Galicia, Santiago de Compostela, Spain.
Clin Biochem. 1991 Apr;24(2):179-83. doi: 10.1016/0009-9120(91)90526-k.
A procedure is proposed for the separation of multiple forms of 5'-nucleotidase (EC 3.1.3.5) by cellulose acetate electrophoresis. The effects of various treatments (wheat-germ lectin, neuraminidase, n-butanol, papain, Triton X-100 and precipitation of LDL and VLDL) on the electrophoretic pattern of 5'-nucleotidase and alkaline phosphatase were studied. In healthy controls, the presence of three fractions with alpha 1, alpha 2 and beta mobilities was observed, the latter being the major one. In different hepatobiliary diseases a close relationship between the presence of high molecular weight alkaline phosphatase and the increase in the ratio Total/beta 5'-nucleotidase was observed, showing that the increase in total 5'-nucleotidase in these patients is mainly due to the alpha 1 isoform. The nature of these forms is discussed.
本文提出了一种通过醋酸纤维素电泳分离5'-核苷酸酶（EC 3.1.3.5）多种形式的方法。研究了各种处理（麦胚凝集素、神经氨酸酶、正丁醇、木瓜蛋白酶、Triton X-100以及低密度脂蛋白和极低密度脂蛋白的沉淀）对5'-核苷酸酶和碱性磷酸酶电泳图谱的影响。在健康对照中，观察到具有α1、α2和β迁移率的三个组分，其中β组分是主要组分。在不同的肝胆疾病中，观察到高分子量碱性磷酸酶的存在与总/β 5'-核苷酸酶比值增加之间存在密切关系，表明这些患者总5'-核苷酸酶的增加主要归因于α1同工型。讨论了这些形式的性质。
