Panteghini M
Laboratorio Analisi Chimico-Cliniche, Spedali Civili, Brescia, Italy.
Clin Chem. 1994 Feb;40(2):190-6.
Human isonucleotidases were separated by electrophoresis on a cellulose acetate membrane. Three 5'-nucleotidase forms, NTP1, NTP2, and NTP3, were resolved with this method and quantified by densitometry. The procedure was not only simple and rapid but also sufficiently precise (between-run CV < 20%) and sensitive (detected nucleotidase fractions of > 0.5 U/L). The effects of various treatments (heat, neuraminidase, glycosidases, proteases, lectins, and detergents) on the electrophoretic pattern of 5'-nucleotidase were studied. NTP1 (mean 12% of total 5'-nucleotidase, SD 5%), NTP2 (mean 30%, SD 8%), and NTP3 (mean 58%, SD 8%) were found in all normal persons studied. The increase in total 5'-nucleotidase in patients with hepatobiliary disease was mainly due to the NTP1 isoform.
通过在醋酸纤维素膜上进行电泳分离人同功核苷酸酶。用该方法分离出三种5'-核苷酸酶形式，即NTP1、NTP2和NTP3，并通过光密度测定法定量。该方法不仅简单快速，而且具有足够的精密度（批间变异系数<20%）和灵敏度（可检测到>0.5 U/L的核苷酸酶组分）。研究了各种处理（加热、神经氨酸酶、糖苷酶、蛋白酶、凝集素和去污剂）对5'-核苷酸酶电泳图谱的影响。在所有研究的正常个体中均发现了NTP1（平均占总5'-核苷酸酶的12%，标准差5%）、NTP2（平均30%，标准差8%）和NTP3（平均58%，标准差8%）。肝胆疾病患者总5'-核苷酸酶的升高主要归因于NTP1同工型。
