College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Theor Appl Genet. 2010 Aug;121(4):643-50. doi: 10.1007/s00122-010-1336-3. Epub 2010 Apr 17.
Cytoplasmic male sterility (CMS) has widely been used as an efficient pollination control system in rapeseed hybrid production. Identification of cytoplasm type of rapeseed accessions is becoming the most important basic work for hybrid-rapeseed breeding. In this study, we report a simple multiplex PCR method to distinguish the existing common cytoplasm resources, Pol, Nap, Cam, Ogu and Ogu-NWSUAF cytoplasm, in rapeseed. Cytoplasm type of 35 F(1) hybrids and 140 rapeseed open pollinated varieties or breeding lines in our rapeseed breeding programme were tested by this method. The results indicated that 10 of 35 F(1) hybrids are the Nap, and 25 the Pol cytoplasm type, which is consistent with the information provided by the breeders. Out of 140 accessions tested, 100 (71.4%), 21 (15%) and 19 (13.6%) accessions possess Nap, Cam and Pol cytoplasm, respectively. All 19 accessions with Pol cytoplasm are from China. Pedigree analysis indicated that these accessions with Pol cytoplasm were either restorers for Pol CMS, including Shaan 2C, Huiyehui, 220, etc. or derived from hybrids with Pol CMS as female parent. Our molecular results are consistent with those of the classical testcross, suggesting the reliability of this method. The multiplex PCR assay method can be applied to CMS "three-line" breeding, selection and validation of hybrid rapeseed.
细胞质雄性不育（CMS）已广泛应用于油菜杂种生产中的高效授粉控制系统。油菜种质细胞质类型的鉴定已成为杂交油菜育种的最重要的基础工作。本研究报道了一种简单的多重 PCR 方法，用于区分油菜中现有的常见细胞质资源，如 Pol、Nap、Cam、Ogu 和 Ogu-NWSUAF 细胞质。利用该方法对 35 个 F1 杂种和 140 个油菜开放授粉品种或自交系的细胞质类型进行了检测。结果表明，35 个 F1 杂种中有 10 个为 Nap 细胞质型，25 个为 Pol 细胞质型，与育种家提供的信息一致。在所检测的 140 个品系中，分别有 100（71.4%）、21（15%）和 19（13.6%）个品系具有 Nap、Cam 和 Pol 细胞质。所有 19 个具有 Pol 细胞质的品系均来自中国。系谱分析表明，这些具有 Pol 细胞质的品系要么是 Pol CMS 的恢复系，包括陕 2C、回掖会、220 等，要么是 Pol CMS 作为母本的杂种后代。我们的分子结果与经典测交结果一致，表明该方法的可靠性。该多重 PCR 检测方法可应用于 CMS“三系”育种、杂交油菜的选择和验证。
