Faculty of Chemistry, Northeast Normal University, Changchun, Jilin, PR China.
Electrophoresis. 2010 May;31(10):1697-705. doi: 10.1002/elps.200900541.
The application of chemical-modified gold nanoparticles (GNPs) as chiral selector for the enantioseparation based on pseudostationary phase-CEC (PSP-CEC) is presented. GNPs modified by thiolated beta-CD were characterized by NMR and FT-IR. The nanoparticle size was determined to be of 9.5 nm (+2.5 nm) by Transmission Electron Microscopy (TEM) and UV spectra. Four pairs of dinitrophenyl-labeled amino acid enantiomers (DL-Val, Leu, Glu and Asp) and three pairs of drug enantiomers (RS-chlorpheniramine, zopiclone and carvedilol) were analyzed by using modified GNPs as the chiral selector in PSP-CEC. Good theoretical plate number (up to 2.4x10(5) per meter) and separation resolution (up to 4.7) were obtained even with low concentration of modified GNPs (0.8-1.4 mg/mL). The corresponding concentration of beta-CD in the buffer was only 0.30-0.53 mM, which was much lower than the optimum concentration of 15 mM if pure beta-CD was used as chiral selector. Our results showed that thiolated beta-CD modified GNPs have more sufficient interaction with the analytes, resulting in significant enhancement of enantioseparation. The study shed light on potential usage of chemical modified GNPs as chiral selector for enantioseparation based on PSP-CEC.
本文介绍了化学修饰的金纳米粒子（GNPs）作为手性选择剂在基于伪稳定相毛细管电泳（PSP-CEC）的对映体分离中的应用。通过 NMR 和 FT-IR 对巯基化 β-CD 修饰的 GNPs 进行了表征。通过透射电子显微镜（TEM）和紫外光谱确定纳米颗粒的尺寸为 9.5nm（+2.5nm）。使用修饰的 GNPs 作为手性选择剂，通过 PSP-CEC 分析了四对二硝基苯标记的氨基酸对映体（DL-Val、Leu、Glu 和 Asp）和三对对映体药物（RS-氯苯那敏、佐匹克隆和卡维地洛）。即使在低浓度的修饰 GNPs（0.8-1.4mg/mL）下，也可以获得良好的理论板数（高达 2.4x10(5)每米）和分离分辨率（高达 4.7）。缓冲液中 β-CD 的相应浓度仅为 0.30-0.53mM，如果使用纯 β-CD 作为手性选择剂，则低于最佳浓度 15mM。我们的结果表明，巯基化 β-CD 修饰的 GNPs 与分析物具有更充分的相互作用，从而显著增强了对映体分离。该研究为基于 PSP-CEC 的手性选择剂在对映体分离中的潜在应用提供了新的思路。
