Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Osaka, Japan.
Biotechnol J. 2010 May;5(5):526-9. doi: 10.1002/biot.200900289.
Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T(m)) of tRNA was calculated from normalized theta(208). Addition of POPC/Ch liposomes decreased the T(m) value of tRNA from 48 degrees C to 38 degrees C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.
生物膜在细胞对热应激的反应中起着重要作用。在这项研究中，我们专注于脂质体和 tRNA 之间的相互作用。在热处理后，我们测定了存在于由 POPC（1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸胆碱）和胆固醇（Ch）制备的脂质体中的 tRNA 的圆二色性光谱。为了比较热稳定性，从归一化的θ（208）计算 tRNA 的中点温度（T（m））。添加 POPC/Ch 脂质体将 tRNA 的 T（m）值从 48°C 降低至 38°C。我们得出结论，POPC/Ch 脂质体与 tRNA 相互作用并在热应激下破坏其构象。
