Rassmann K, Schlötterer C, Tautz D
Institut für Genetik und Mikrobiologie, Universität München, Germany.
Electrophoresis. 1991 Feb-Mar;12(2-3):113-8. doi: 10.1002/elps.1150120205.
Simple sequences are short regions of tandem repetitions of mono-, di-, tri-, or tetranucleotide motifs and occur as repetitive elements in all eukaryotic genomes. These regions tend to be hypervariable in length and can therefore be exploited for DNA fingerprinting purposes, using the polymerase chain reaction with primers flanking such regions. We describe how suitable simple-sequence loci can be isolated from any given eukaryotic DNA. We show the DNA sequences for a number of variants of such loci and discuss the current results on their usefulness for DNA fingerprinting.
简单序列是单核苷酸、二核苷酸、三核苷酸或四核苷酸基序串联重复的短区域，作为重复元件存在于所有真核生物基因组中。这些区域的长度往往高度可变，因此可利用聚合酶链反应（PCR），使用位于这些区域两侧的引物，将其用于DNA指纹识别。我们描述了如何从任何给定的真核生物DNA中分离出合适的简单序列位点。我们展示了多个此类位点变体的DNA序列，并讨论了目前关于它们在DNA指纹识别方面有用性的研究结果。
