Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
Pathol Int. 2010 Apr;60(4):268-77. doi: 10.1111/j.1440-1827.2010.02512.x.
Given that previous studies indicated that early growth response 1 (EGR1) exerts pro-tumorigenic effects through regulating heparanase (HPA) transcription, it was hypothesized that EGR1 may correlate with the progression of gastric cancer. One hundred and fifteen patients with gastric cancer were evaluated for the protein and transcript expression of EGR1 and HPA on immunohistochemistry and real-time quantitative polymerase chain reaction (PCR). In normal gastric mucosa, EGR1 protein expression was absent or weak, whereas gastric cancer was positive for EGR1. Seventy gastric cancer patients (60.9%) were positive for cytoplasmic EGR1 expression, and 26 (22.6%) had nuclear expression of EGR1. In the gastric cancer examined, the transcripts of EGR1 were enhanced compared to that of normal gastric mucosa, and positively correlated with EGR1 protein expression. The cytoplasmic or nuclear expression of EGR1 and its transcripts in gastric cancer was positively correlated with tumor infiltration (P < 0.05), lymph node and distant metastasis (P < 0.05), tumor node metastasis (TNM) stages (P < 0.05), but not with age, gender, tumor location and size, histological types or differentiation. Moreover, the protein and transcript expression of EGR1 was correlated with that of HPA in gastric cancer. These results indicate that aberrant expression of EGR1 in gastric cancer is associated with tumor invasion and metastasis, and HPA transcription.
鉴于先前的研究表明，早期生长反应 1（EGR1）通过调节乙酰肝素酶（HPA）转录发挥促肿瘤作用，因此假设 EGR1 可能与胃癌的进展相关。对 115 例胃癌患者进行免疫组织化学和实时定量聚合酶链反应（PCR）检测 EGR1 和 HPA 的蛋白和转录表达。在正常胃黏膜中，EGR1 蛋白表达缺失或微弱，而胃癌呈 EGR1 阳性。70 例胃癌患者（60.9%）细胞质 EGR1 表达阳性，26 例（22.6%）核 EGR1 表达阳性。在检测到的胃癌中，EGR1 的转录物与正常胃黏膜相比增强，并且与 EGR1 蛋白表达呈正相关。胃癌中 EGR1 的细胞质或核表达及其转录物与肿瘤浸润（P<0.05）、淋巴结和远处转移（P<0.05）、肿瘤淋巴结转移（TNM）分期（P<0.05）呈正相关，但与年龄、性别、肿瘤位置和大小、组织学类型或分化无关。此外，EGR1 的蛋白和转录物表达与胃癌中 HPA 的表达相关。这些结果表明，胃癌中 EGR1 的异常表达与肿瘤侵袭和转移以及 HPA 转录有关。
