Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
J Virol Methods. 2010 Sep;168(1-2):18-21. doi: 10.1016/j.jviromet.2010.04.009. Epub 2010 Apr 18.
This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes.
本研究描述了一种新型的基于阻断微球的免疫分析方法，用于高度敏感和特异性地检测牛病毒性腹泻病毒 (BVDV) 的抗体。该方法的内和间分析变异系数分别为 4.9%和小于 7%，而珠结合的变异性小于 6.6%。通过检测总共 509 份血清样本，评估了该检测方法的诊断性能。基于阴性/阳性截断值为 30.3%，该检测方法相对于 ELISA 的灵敏度为 99.4%，特异性为 98.3%。新型微球免疫分析为传统 ELISA 系统提供了一种替代方法，可用于 BVD 控制计划中的高通量筛选。
