College of Resource and Environmental Sciences, Nanjing Agricultural University, Weigang Road 1, Nanjing 210095, China.
Bioresour Technol. 2010 Sep;101(18):6895-901. doi: 10.1016/j.biortech.2010.03.122. Epub 2010 Apr 18.
The arbuscular mycorrhizal (AM) hyphae-mediated uptake of polycyclic aromatic hydrocarbons (PAHs) by the roots of ryegrass (Lolium multiflorum Lam.) was investigated using three-compartment systems. Glomus mosseae and Glomus etunicatum were chosen, and fluorene and phenanthrene were used as representative PAHs. When roots were grown in un-spiked soils, AM hyphae extended into PAH-spiked soil and clearly absorbed and transported PAHs to roots, resulting in high concentrations of fluorene and phenanthrene in roots. This was further confirmed by the batch equilibration experiment, which revealed that the partition coefficients (K(d)) of tested PAHs by mycorrhizal hyphae were 270-356% greater than those by roots, suggesting the great potential of hyphae to absorb PAHs. Because of fluorene's lower molecular weight and higher water solubility, its translocation by hyphae was greater than that of phenanthrene. These results provide new perspectives on the AM hyphae-mediated uptake by plants of organic contaminants from soil.
采用三隔室系统研究了丛枝菌根（AM）菌丝介导黑麦草（Lolium multiflorum Lam.）根系对多环芳烃（PAHs）的吸收。选择摩西球囊霉和厚垣轮枝孢菌，并将荧蒽和菲作为代表性的 PAHs。当根系在未添加污染物的土壤中生长时，AM 菌丝延伸到添加了 PAHs 的土壤中，并明显吸收和将 PAHs 运输到根系，导致根系中荧蒽和菲的浓度很高。这通过批平衡实验进一步得到证实，该实验表明，被菌根菌丝吸收的测试 PAHs 的分配系数（K(d)）比根系高 270-356%，这表明菌丝吸收 PAHs 的巨大潜力。由于荧蒽的分子量较低且水溶性较高，因此其菌丝的转运量大于菲。这些结果为丛枝菌根菌丝介导植物从土壤中吸收有机污染物提供了新的视角。
