变应原特异性分泌白细胞介素-10 的 I 型 T 调节细胞，而不是 CD4+CD25+Foxp3+T 细胞，在持续性变应性鼻炎患者的外周血中减少。

Allergen-specific IL-10-secreting type I T regulatory cells, but not CD4(+)CD25(+)Foxp3(+) T cells, are decreased in peripheral blood of patients with persistent allergic rhinitis.

机构信息

Department of Otolaryngology, Beijing TongRen Hospital, Capital Medical University, Beijing, China.

出版信息

Clin Immunol. 2010 Aug;136(2):292-301. doi: 10.1016/j.clim.2010.03.006. Epub 2010 Apr 18.

DOI:10.1016/j.clim.2010.03.006

PMID:20403730

Abstract

We investigate the frequencies of CD4(+)CD25(+)Foxp3(+) T cells and allergen-specific IL-10(+)IL-4(-), IFN-gamma(+)IL-4(-), IL-4(+)IFN-gamma(-)CD4(+) T cells (which display characteristics of nTreg, Tr1-, Th1- and Th2- cells, respectively) in peripheral blood mononuclear cells (PBMCs) of patients with AR and of healthy individuals. In addition, we estimated the suppressive effect of CD4(+)CD25(+) Treg cells and allergen-specific, IL-10-secreting cells from both two groups. The frequency of CD4(+)CD25(+)Foxp3(+) T cells is similar in 43 AR patients compared with 38 healthy subjects. CD4(+)CD25(high) cells retain suppressive activity on allergen-stimulated cell proliferation and cytokine production of Th1 but not Th2 cells in both groups. However, the frequency of allergen-specific IL-10(+)IL-4(-)CD4(+) T cells is reduced in AR patients, and correlates inversely to clinical symptom scores. Allergen-specific, IL-10-secreting cells potently suppressed D. pteronyssinus major allergen 1-stimulated cell proliferation and cytokine production (IFN-gamma and IL-4) in healthy individuals. Altogether our data indicate that the number and function of CD4(+)CD25(+) Treg cells from allergic patients are not impaired. However, the deficiency of allergen-specific Tr1 cells may play a role in the development of AR.

摘要

我们研究了外周血单个核细胞（PBMCs）中 AR 患者和健康个体中 CD4(+)CD25(+)Foxp3(+)T 细胞以及过敏原特异性的 IL-10(+)IL-4(-)、IFN-gamma(+)IL-4(-)、IL-4(+)IFN-gamma(-)CD4(+)T 细胞（分别显示 nTreg、Tr1、Th1 和 Th2 细胞的特征）的频率。此外，我们还估计了两组 CD4(+)CD25(+)Treg 细胞和过敏原特异性、IL-10 分泌细胞的抑制作用。43 例 AR 患者与 38 例健康个体的 CD4(+)CD25(+)Foxp3(+)T 细胞频率相似。CD4(+)CD25(high)细胞在两组中对过敏原刺激的细胞增殖和 Th1 细胞因子产生均具有抑制活性，但对 Th2 细胞无抑制活性。然而，AR 患者过敏原特异性的 IL-10(+)IL-4(-)CD4(+)T 细胞频率降低，与临床症状评分呈负相关。过敏原特异性、IL-10 分泌细胞可有效抑制 D. pteronyssinus major 过敏原 1 刺激的细胞增殖和细胞因子产生（IFN-gamma 和 IL-4），在健康个体中。总之，我们的数据表明，过敏患者的 CD4(+)CD25(+)Treg 细胞数量和功能没有受损。然而，过敏原特异性 Tr1 细胞的缺乏可能在 AR 的发生中起作用。