Genc Sema, Eroglu Hacer, Kucuksezer Umut Can, Aktas-Cetin Esin, Gelincik Asli, Ustyol-Aycan Esra, Buyukozturk Suna, Deniz Gunnur
Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul University, Istanbul, Turkey.
J Asthma. 2012 Aug;49(6):569-74. doi: 10.3109/02770903.2012.695418.
Regulatory (CD4(+)CD25(+)) T cells have been shown to play an important role in the development of allergic diseases. This study aims to investigate CD4(+)CD25(+) T cells, Forkhead box P3 (FoxP3(+) cells), and T-helper 1/T-helper 2 (Th1/Th2) cytokines in newly diagnosed allergic rhinitis (AR) patients.
Altogether, 10 subjects with AR and 12 age-matched nonallergic healthy subjects were included in this study. CD4(+)CD25(+) T cells, FoxP3(+) T cells in peripheral blood mononuclear cells (PBMCs) were evaluated by flow cytometry, and the Th1/Th2 cytokine levels were determined by cytometric bead array immunoassay in both PBMC supernatants and nasal lavage fluids.
The percentage of CD4(+)CD25(+) T cells were significantly higher, whereas the percentage of FoxP3(+) cells were lower in AR patients compared with healthy subjects. In PBMC culture supernatants, interleukin-10 (IL-10) levels were significantly lower (p = .012), whereas IL-4, IL-5, and tumor necrosis factor-α (TNF-α) levels in nasal lavage fluids were higher in AR patients compared with healthy subjects (p = .026, p = .015, p = .03, respectively).
Our findings indicate that decrease in CD4(+)CD25(+)FoxP3(+) T cell fraction and diminished levels of IL-10 are noteworthy without allergen stimulation in house dust mite AR patients.
调节性（CD4(+)CD25(+)）T细胞已被证明在过敏性疾病的发展中起重要作用。本研究旨在调查新诊断的变应性鼻炎（AR）患者中的CD4(+)CD25(+) T细胞、叉头框蛋白P3（FoxP3(+)细胞）和辅助性T细胞1/辅助性T细胞2（Th1/Th2）细胞因子。
本研究共纳入10例AR患者和12例年龄匹配的非过敏性健康受试者。采用流式细胞术评估外周血单个核细胞（PBMC）中的CD4(+)CD25(+) T细胞、FoxP3(+) T细胞，并通过细胞计数珠阵列免疫测定法测定PBMC上清液和鼻腔灌洗液中的Th1/Th2细胞因子水平。
与健康受试者相比，AR患者中CD4(+)CD25(+) T细胞的百分比显著更高，而FoxP3(+)细胞的百分比更低。在PBMC培养上清液中，白细胞介素10（IL-10）水平显著更低（p = 0.012），而与健康受试者相比，AR患者鼻腔灌洗液中的IL-4、IL-5和肿瘤坏死因子-α（TNF-α）水平更高（分别为p = 0.026、p = 0.015、p = 0.03）。
我们的研究结果表明，在屋尘螨AR患者中，未经过敏原刺激时，CD4(+)CD25(+)FoxP3(+) T细胞比例降低和IL-10水平降低值得关注。
Zotero 插件