Cai Jing, Wang Fang, Shi Sheng-Liu
Department of Octolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming, People's Republic of China.
Department of Rehabilitation Medicine, First Affiliated Hospital of Kunming Medical University, Kunming, People's Republic of China.
Kaohsiung J Med Sci. 2025 Jan;41(1):e12918. doi: 10.1002/kjm2.12918. Epub 2024 Dec 11.
The dysregulation of microRNA (miRNA) expression contributes to the development of allergic rhinitis (AR). This study investigates serum miR-135a levels and their association with regulatory T cell (Treg) and T helper cell 17 (Th17) balance in AR patients. A total of 93 AR patients and 76 healthy controls were retrospectively recruited. Levels of serum miR-135a, peripheral blood Th17 and Treg cells, and Treg/Th17-related cytokines were measured. We assessed the diagnostic value of serum miR-135a for AR and its relationship with Treg/Th17 balance. AR patients showed significantly elevated immunoglobulin E (IgE), peripheral blood Th17 cells, and IL-17 and IL-6 levels, alongside reduced serum miR-135a, Treg cells, IL-10, TGF-β1, and Treg/Th17 ratios. A serum miR-135a of ≤0.536 demonstrated diagnostic potential for AR. Patients with higher serum miR-135a levels displayed increased Treg cell level and Treg/Th17 ratios, reduced Th17 cell, and lower total nasal symptom score (TNSS). Serum miR-135a levels in AR patients negatively correlated with TNSS, IL-17, IL-6, and Th17 cell percentages, and positively correlated with IL-10, TGF-β1, Treg cell percentages, and Treg/Th17 ratios. Collectively, decreased serum miR-135a levels in AR patients are associated with Treg/Th17 balance, supporting miR-135a as a potential biomarker for AR diagnosis.
微小RNA（miRNA）表达失调促进了变应性鼻炎（AR）的发展。本研究调查了AR患者血清miR-135a水平及其与调节性T细胞（Treg）和辅助性T细胞17（Th17）平衡的关系。回顾性招募了93例AR患者和76名健康对照。检测血清miR-135a、外周血Th17和Treg细胞以及Treg/Th17相关细胞因子的水平。我们评估了血清miR-135a对AR的诊断价值及其与Treg/Th17平衡的关系。AR患者的免疫球蛋白E（IgE）、外周血Th17细胞以及IL-17和IL-6水平显著升高，同时血清miR-135a、Treg细胞、IL-10、TGF-β1以及Treg/Th17比值降低。血清miR-135a≤0.536对AR具有诊断潜力。血清miR-135a水平较高的患者Treg细胞水平和Treg/Th17比值升高，Th17细胞减少，总鼻症状评分（TNSS）较低。AR患者血清miR-135a水平与TNSS、IL-17、IL-6以及Th17细胞百分比呈负相关，与IL-10、TGF-β1、Treg细胞百分比以及Treg/Th17比值呈正相关。总体而言，AR患者血清miR-135a水平降低与Treg/Th17平衡相关，支持miR-135a作为AR诊断的潜在生物标志物。
