Max F. Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria.
Cell Cycle. 2010 May;9(9):1802-8. doi: 10.4161/cc.9.9.11526. Epub 2010 May 15.
Meiosis is the process which produces haploid gametes from diploid precursor cells. This reduction of chromosome number is achieved by two successive divisions. Whereas homologs segregate during meiosis I, sister chromatids segregate during meiosis II. To identify novel proteins required for proper segregation of chromosomes during meiosis, we applied a high-throughput knockout technique to delete 87 S. pombe genes whose expression is upregulated during meiosis and analyzed the mutant phenotypes. Using this approach, we identified a new protein, Dil1, which is required to prevent meiosis I homolog non-disjunction. We show that Dil1 acts in the dynein pathway to promote oscillatory nuclear movement during meiosis.
减数分裂是一个将二倍体前体细胞转化为单倍体配子的过程。这一染色体数目的减少是通过两次连续的分裂实现的。在减数分裂 I 中同源染色体分离，而在减数分裂 II 中姐妹染色单体分离。为了鉴定在减数分裂过程中正确分离染色体所必需的新蛋白质，我们应用了一种高通量的敲除技术来删除 87 个在减数分裂中表达上调的 S. pombe 基因，并分析了突变表型。通过这种方法，我们鉴定了一种新的蛋白质 Dil1，它可以防止减数分裂 I 中同源染色体的不分离。我们发现 Dil1 在驱动蛋白途径中发挥作用，以促进减数分裂过程中的核振荡运动。
