School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China.
J Sep Sci. 2010 Jun;33(11):1587-94. doi: 10.1002/jssc.200900866.
A sensitive and reliable HPLC-diode-array detector method was developed for the first time to simultaneously determine nine nucleosides and nucleobases including uracil, cytidine, guanine, uridine, thymine, inosine, guanosine, thymidine and adenosine in 13 different Fritillaria species. The analysis was performed on a BaseLine C18 column with a gradient of acetonitrile in water at a flow rate of 0.8 mL/min. The diode-array detector wavelength was set at 260 nm for the UV detection of nucleosides and nucleobases. Satisfactory separation of these compounds was obtained in less than 40 min. The optimized method provided good linear relation (r(2)>0.9995 for all the investigated analytes), satisfactory precision (RSD <1.51%) and good recovery (from 97.64 to 101.16%). The established method was successfully applied to simultaneous determination of nine nucleosides and nucleobases in 61 batches of samples from 13 Fritillaria species collected from different habitats in China, which could be helpful to control the quality of Fritillaria bulbs.
首次建立了同时测定 13 种贝母属植物中 9 种核苷和碱基（尿嘧啶、胞嘧啶、鸟嘌呤、尿苷、胸腺嘧啶、次黄嘌呤、鸟苷、胸苷和腺苷）的高效液相色谱-二极管阵列检测法。分析采用 BaseLine C18 柱，以乙腈-水为流动相进行梯度洗脱，流速为 0.8 mL/min，二极管阵列检测器的检测波长设定为 260nm，用于检测核苷和碱基。在不到 40min 的时间内实现了这些化合物的良好分离。该优化方法具有良好的线性关系（所有分析物的 r(2)>0.9995）、令人满意的精密度（RSD <1.51%）和良好的回收率（97.64%~101.16%）。该方法成功应用于 61 批来自中国不同产地的 13 种贝母属植物样品中 9 种核苷和碱基的同时测定，有助于控制贝母鳞茎的质量。
