Department of Chemistry, Saint Louis University, 3501 Laclede Avenue, St. Louis, Missouri 63103, USA.
J Am Chem Soc. 2010 May 12;132(18):6288-9. doi: 10.1021/ja101326b.
Living cells oxidize a wide variety of fuels by employing enzymes as catalysts for energy conversion. It has been shown that many of these cellular metabolic enzymes exist in sequential and proximal organization within living organisms. This organization, called a metabolon, results in channeling of substrates between enzymes. Biofuel cell researchers have studied multienzyme systems, but they have not investigated the need for proximal three-dimension organization in efficient energy conversion. This work outlines the use of a Krebs cycle metabolon catalyst obtained through the in situ cross-linking of protein within the mitochondria of Saccharomyces cervisea. This cross-linking maintains the integrity of the sequential complexation that exists naturally in the intact biological system. These complexes channel substrate through the enzyme cascade and improve mass transport, thus increasing the current and power density of pyruvate/air enzymatic biofuel cells.
活细胞通过利用酶作为能量转换的催化剂来氧化多种燃料。已经表明，许多这些细胞代谢酶在生物体中以连续和邻近的组织形式存在。这种组织称为代谢物，导致酶之间的底物定向输送。生物燃料电池研究人员已经研究了多酶系统，但他们没有研究在有效能量转换中对邻近三维组织的需求。这项工作概述了通过在酿酒酵母的线粒体中使蛋白质原位交联获得的三羧酸循环代谢物催化剂的用途。这种交联保持了在完整的生物系统中天然存在的顺序复合物的完整性。这些复合物通过酶级联引导底物，并改善质量传递，从而提高丙酮酸/空气酶促生物燃料电池的电流和功率密度。
