Instituto Tecnológico y de Estudios Superiores de Occidente A. C. (ITESO), Periférico Sur 8585, Tlaquepaque, Jalisco, México.
Lett Appl Microbiol. 2010 Jun 1;50(6):585-90. doi: 10.1111/j.1472-765X.2010.02837.x. Epub 2010 Mar 17.
To study how the antimicrobial and antioxidant activities of Lippia graveolens essential oils with different composition are affected after the microencapsulation process with beta-cyclodextrin (beta CD).
Three Mexican oregano essential oils (EOs) with different carvacrol/thymol/p-cymene ratios (38 : 3 : 32, 23 : 2 : 42, 7 : 19 : 35) were used in this study. Microencapsulation was carried out by spray-drying. Antimicrobial activities were measured as MBC (minimal bactericidal concentration) using 0.05%/0.10%/0.20% (w/v) dilutions of EOs against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 9027 and Staphylococcus aureus ATCC 6538. Antioxidant activities were determined by the 2,2'-diphenyl-1-picrylhydrazil (DPPH) method. EOs showed antimicrobial and antioxidant activity, but microencapsulation preserved the antimicrobial activity in all cases and increased the antioxidant activity from four- to eightfold.
Although the Lippia essential oils were from the same species, their composition affects the biological activities before and after the microencapsulation process, as well as encapsulation efficiency. Our study supports the fact that microencapsulation of EOs in beta-cyclodextrin preserves the antimicrobial activity, improves the antioxidant activity and acts as a protection for EOs main compounds.
Microencapsulation affects positively EOs main compounds, improves antioxidant activity and retains antimicrobial activity, enhancing the quality of the oils.
研究不同组成的 Lippia graveolens 精油在经过β-环糊精（β-CD）微胶囊化处理后，其抗菌和抗氧化活性的变化。
本研究使用了三种具有不同香芹酚/百里酚/对伞花烃比例的墨西哥牛至精油（EOs）（38:3:32、23:2:42、7:19:35）。通过喷雾干燥进行微胶囊化。使用 0.05%/0.10%/0.20%（w/v）的 EO 稀释液，测定最小杀菌浓度（MBC），以评估 EOs 对大肠杆菌 ATCC 11229、铜绿假单胞菌 ATCC 9027 和金黄色葡萄球菌 ATCC 6538 的抗菌活性。通过 2,2'-二苯基-1-苦基肼（DPPH）法测定抗氧化活性。EOs 表现出抗菌和抗氧化活性，但在所有情况下，微胶囊化都保留了抗菌活性，并将抗氧化活性提高了 4 到 8 倍。
尽管 Lippia 精油来自同一物种，但它们的组成会影响微胶囊化前后的生物活性，以及包封效率。我们的研究支持了这样一个事实，即在β-环糊精中微囊化 EOs 可以保留抗菌活性，提高抗氧化活性，并保护 EOs 的主要化合物。
微胶囊化对 EOs 的主要化合物产生积极影响，提高了抗氧化活性，并保留了抗菌活性，从而提高了油的质量。
